ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQSSRLSAL PIFQASLSAS QSPRYIFSSQ NGTRIVFIQD NIIRWYNVLT DSLYHSLNFS 

        70         80         90        100        110        120 
RHLVLDDTFH VISSTSGDLL CLFNDNEIFV MEVPWGYSNV EDVSIQDAFQ IFHYSIDEEE 

       130        140        150        160        170        180 
VGPKSSIKKV LFHPKSYRDS CIVVLKEDDT ITMFDILNSQ EKPIVLNKPN NSFGLDARVN 

       190        200        210        220        230        240 
DITDLEFSKD GLTLYCLNTT EGGDIFAFYP FLPSVLLLNE KDLNLILNKS LVMYESLDST 

       250        260        270        280        290        300 
TDVIVKRNVI KQLQFVSKLH ENWNSRFGKV DIQKEYRLAK VQGPFTINPF PGELYDYTAT 

       310        320        330        340        350        360 
NIATILIDNG QNEIVCVSFD DGSLILLFKD LEMSMSWDVD NYVYNNSLVL IERVKLQREI 

       370        380        390        400        410        420 
KSLITLPEQL GKLYVISDNI IQQVNFMSWA STLSKCINES DLNPLAGLKF ESKLEDIATI 

       430        440        450        460        470        480 
ERIPNLAYIN WNDQSNLALM SNKTLTFQNI SSDMKPQSTA AETSISTEKS DTVGDGFKMS 

       490        500        510        520        530        540 
FTQPINEILI LNDNFQKACI SPCERIIPSA DRQIPLKNEA SENQLEIFTD ISKEFLQRIV 

       550        560        570        580        590        600 
KAQTLGVSIH NRIHEQQFEL TRQLQSTCKI ISKDDDLRRK FEAQNKKWDA QLSRQSELME 

       610        620        630        640        650        660 
RFSKLSKKLS QIAESNKFKE KKISHGEMKW FKEIRNQILQ FNSFVHSQKS LQQDLSYLKS 

       670        680        690        700        710 
ELTRIEAETI KVDKKSQNEW DELRKMLEID SKIIKECNEE LLQVSQEFTT KTQ 

References and documentation are available.

Number of amino acids: 713

Molecular weight: 82085.39

Theoretical pI: 5.22

Amino acid composition: 
Ala (A)  24	  3.4%
Arg (R)  25	  3.5%
Asn (N)  48	  6.7%
Asp (D)  47	  6.6%
Cys (C)   9	  1.3%
Gln (Q)  43	  6.0%
Glu (E)  51	  7.2%
Gly (G)  19	  2.7%
His (H)  10	  1.4%
Ile (I)  64	  9.0%
Leu (L)  76	 10.7%
Lys (K)  54	  7.6%
Met (M)  13	  1.8%
Phe (F)  38	  5.3%
Pro (P)  20	  2.8%
Ser (S)  75	 10.5%
Thr (T)  35	  4.9%
Trp (W)   9	  1.3%
Tyr (Y)  17	  2.4%
Val (V)  36	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 98
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      3665
Hydrogen    H	      5792
Nitrogen    N	       962
Oxygen      O	      1128
Sulfur      S	        22

Formula: C3665H5792N962O1128S22
Total number of atoms: 11569

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    75330
Abs 0.1% (=1 g/l)   0.918, assuming all pairs of Cys residues form cystines


Ext. coefficient    74830
Abs 0.1% (=1 g/l)   0.912, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.52
This classifies the protein as unstable.



Aliphatic index: 94.59

Grand average of hydropathicity (GRAVY): -0.345