ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQVIEPQLD RTTYYSILGL TSNATSSEVH KSYLKLARLL HPDKTKSDKS EELFKAVVHA
70 80 90 100 110 120
HSILTDEDQK LRYDRDLKIK GLHTYQPKKN CHIFKTKAKE SQGASPTLGQ SEAYHRQNKP
130 140 150 160 170 180
YEQQPYGFGV GKKMTSSSKS KVPIFKSFNL KSYQRNHYYS SKKERKHGSP DIDSLFHETN
190 200 210 220 230 240
GASKVRMTDA GKMDTNSQFQ EIWEILGKNA YTHKSYSEDP NSCLGSALSD HEEEEEAGKQ
250 260 270 280 290 300
QQQQQQQQQQ QQHYGMTSKS SSPDEEKKNN KEPKRESRVS PEENGEEETG HKQFKLPKTS
310 320 330 340 350 360
TFSSGSHDSN LQSPFYNHEY RHYARSKFEC KNQFRKSVSP IKEIPATTSA NEGWNILRDI
370 380 390 400 410 420
IEKLNISNVD DRNKDLLFRR DEIGDKNHSD SIDIENLSIK EPKGMKRRKK DDISLEELFQ
430 440 450 460 470 480
SLPREKDYFM MDAINDSLES INLFKKPKTT QSHEQGGTFA QAESNRAKFK PLLEQCGITP
490 500 510 520 530 540
EILDLEIPEI PEFDAVADLE TLKLNVQLFN NQCNKLKETI HQVSLQRLRA DTQFSDMLTQ
550 560 570 580
KQSIMVWKTY LEFDKSLMDK LNILQERQMQ VIKIFSERCD GKV
References and
documentation are available.
Number of amino acids: 583
Molecular weight: 67389.47
Theoretical pI: 7.98
Total number of negatively charged residues (Asp + Glu): 87
Total number of positively charged residues (Arg + Lys): 89
Atomic composition:
Carbon C 2944
Hydrogen H 4654
Nitrogen N 838
Oxygen O 939
Sulfur S 18
Formula: C
2944H
4654N
838O
939S
18
Total number of atoms: 9393
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45185
Abs 0.1% (=1 g/l) 0.671, assuming all pairs of Cys residues form cystines
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 0.665, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 60.58
This classifies the protein as unstable.
Aliphatic index: 63.24
Grand average of hydropathicity (GRAVY): -1.038