ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQVQEQHIS ESQLQYGNGS LMSTVPADLS QSVVDGNGNG SSEDIEATNG SGDGGGLQEQ 

        70         80         90        100        110        120 
AEAQGEMEDE AYDEAALGSF VPIEKLQVNG ITMADVKKLR ESGLHTAEAV AYAPRKDLLE 

       130        140        150        160        170        180 
IKGISEAKAD KLLNEAARLV PMGFVTAADF HMRRSELICL TTGSKNLDTL LGGGVETGSI 

       190        200        210        220        230        240 
TELFGEFRTG KSQLCHTLAV TCQIPLDIGG GEGKCLYIDT EGTFRPVRLV SIAQRFGLDP 

       250        260        270        280        290        300 
DDALNNVAYA RAYNADHQLR LLDAAAQMMS ESRFSLIVVD SVMALYRTDF SGRGELSARQ 

       310        320        330        340        350        360 
MHLAKFMRAL QRLADQFGVA VVVTNQVVAQ VDGGMAFNPD PKKPIGGNIM AHSSTTRLGF 

       370        380        390        400 
KKGKGCQRLC KVVDSPCLPE AECVFAIYED GVGDPREEDE 

References and documentation are available.

Number of amino acids: 400

Molecular weight: 42963.44

Theoretical pI: 4.80

Amino acid composition: 
Ala (A)  39	  9.8%
Arg (R)  20	  5.0%
Asn (N)  13	  3.2%
Asp (D)  27	  6.8%
Cys (C)   8	  2.0%
Gln (Q)  21	  5.2%
Glu (E)  30	  7.5%
Gly (G)  41	 10.2%
His (H)   7	  1.8%
Ile (I)  16	  4.0%
Leu (L)  38	  9.5%
Lys (K)  17	  4.2%
Met (M)  13	  3.2%
Phe (F)  14	  3.5%
Pro (P)  13	  3.2%
Ser (S)  27	  6.8%
Thr (T)  19	  4.8%
Trp (W)   0	  0.0%
Tyr (Y)   8	  2.0%
Val (V)  29	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1856
Hydrogen    H	      2973
Nitrogen    N	       525
Oxygen      O	       603
Sulfur      S	        21

Formula: C1856H2973N525O603S21
Total number of atoms: 5978

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12420
Abs 0.1% (=1 g/l)   0.289, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.277, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.37
This classifies the protein as stable.



Aliphatic index: 83.42

Grand average of hydropathicity (GRAVY): -0.218