ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQVQSPLTA TNSGLAVNNN TMNSQMPNRS NVRLVNGTLP PSLHVSSNLN HNTGNSSASY 

        70         80         90        100        110        120 
SGSQSRDDST IVGLHYKIGK KIGEGSFGVL FEGTNMINGL PVAIKFEPRK TEAPQLKDEY 

       130        140        150        160        170        180 
RTYKILAGTP GIPQEYYFGQ EGLHNILVID LLGPSLEDLF DWCGRRFSVK TVVQVAVQMI 

       190        200        210        220        230        240 
TLIEDLHAHD LIYRDIKPDN FLIGRPGQPD ANKVHLIDFG MAKQYRDPKT KQHIPYREKK 

       250        260        270        280        290        300 
SLSGTARYMS INTHLGREQS RRDDMEAMGH VFFYFLRGQL PWQGLKAPNN KQKYEKIGEK 

       310        320        330        340        350        360 
KRLTNVYDLA QGLPIQFGRY LEIVRNLSFE ETPDYEGYRM LLLSVLDDLG ETADGQYDWM 

       370        380        390        400        410        420 
KLNGGRGWDL SINKKPNLHG YGHPNPPNEK SKRHRSKNHQ YSSPDHHHHY NQQQQQQQAQ 

       430        440        450        460        470        480 
AQAQAQAQAK VQQQQLQQAQ AQQQANRYQL QPDDSHYDEE REASKLDPTS YEAYQQQTQQ 

       490        500        510        520        530        540 
KYAQQQQKQM QQKSKQFANT GANGQTNKYP YNAQPTANDE QNAKNAAQDR NSNKSSKGFF 


SKLGCC 

References and documentation are available.

Number of amino acids: 546

Molecular weight: 62079.17

Theoretical pI: 9.10

Amino acid composition: 
Ala (A)  34	  6.2%
Arg (R)  26	  4.8%
Asn (N)  39	  7.1%
Asp (D)  29	  5.3%
Cys (C)   3	  0.5%
Gln (Q)  61	 11.2%
Glu (E)  25	  4.6%
Gly (G)  40	  7.3%
His (H)  19	  3.5%
Ile (I)  21	  3.8%
Leu (L)  44	  8.1%
Lys (K)  38	  7.0%
Met (M)  12	  2.2%
Phe (F)  16	  2.9%
Pro (P)  28	  5.1%
Ser (S)  37	  6.8%
Thr (T)  23	  4.2%
Trp (W)   4	  0.7%
Tyr (Y)  27	  4.9%
Val (V)  20	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2715
Hydrogen    H	      4222
Nitrogen    N	       804
Oxygen      O	       842
Sulfur      S	        15

Formula: C2715H4222N804O842S15
Total number of atoms: 8598

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    62355
Abs 0.1% (=1 g/l)   1.004, assuming all pairs of Cys residues form cystines


Ext. coefficient    62230
Abs 0.1% (=1 g/l)   1.002, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.05
This classifies the protein as unstable.



Aliphatic index: 63.28

Grand average of hydropathicity (GRAVY): -0.967