ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSQYGFVRVP REVEKAIPVV NAPRPRAVVP PPNSETARLV REYAAKELTA PVLNHSLRVF 

        70         80         90        100        110        120 
QYSVAIIRDQ FPAWDLDQEV LYVTCLLHDI ATTDKNMRAT KMSFEYYGGI LSRELVFNAT 

       130        140        150        160        170        180 
GGNQDYADAV TEAIIRHQDL TGTGYITTLG LILQIATTLD NVGSNTDLIH IDTVSAINEQ 

       190        200        210        220 
FPRLHWLSCF ATVVDTENSR KPWGHTSSLG DDFSKKVICN TFGYN 

References and documentation are available.

Number of amino acids: 225

Molecular weight: 25152.48

Theoretical pI: 5.66

Amino acid composition: 
Ala (A)  18	  8.0%
Arg (R)  13	  5.8%
Asn (N)  12	  5.3%
Asp (D)  14	  6.2%
Cys (C)   3	  1.3%
Gln (Q)   8	  3.6%
Glu (E)  11	  4.9%
Gly (G)  12	  5.3%
His (H)   6	  2.7%
Ile (I)  14	  6.2%
Leu (L)  19	  8.4%
Lys (K)   7	  3.1%
Met (M)   3	  1.3%
Phe (F)   9	  4.0%
Pro (P)  11	  4.9%
Ser (S)  13	  5.8%
Thr (T)  20	  8.9%
Trp (W)   3	  1.3%
Tyr (Y)   9	  4.0%
Val (V)  20	  8.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	      1124
Hydrogen    H	      1752
Nitrogen    N	       306
Oxygen      O	       338
Sulfur      S	         6

Formula: C1124H1752N306O338S6
Total number of atoms: 3526

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30035
Abs 0.1% (=1 g/l)   1.194, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   1.189, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.53
This classifies the protein as stable.



Aliphatic index: 90.98

Grand average of hydropathicity (GRAVY): -0.150