ProtParam
User-provided sequence:
10 20 30 40 50 60
MSQYIKCYGR VRPSARLGAS SESLVVGGRV VTIDTDNGSK RYELHHIFDS SSSQEDVFGT
70 80 90 100 110 120
VAKKIVEDCV EGYNGTVFAY GQTGSGKTHT MLGPCDSWTD PELMGLIPRS VEHVFQHLDT
130 140 150 160 170 180
KAKKCQKLTF SVSVEFVELY NEVIYDLLNA KNKVQLRDSG KDIQLVGALP KNVDNPLDLM
190 200 210 220 230 240
HLVQKGWQER STGSTAMNAE SHALLIIRIK TQERTGELVK ERSSILNLVD LAGSERQTHT
250 260 270 280 290 300
KSSGDRLKEA TNINSSLTVL GRCIRLLADP SKAKGHVPYR DSHLTHILKN SLGGNSKTAV
310 320 330 340 350 360
IVNMHPDRDF AQESNSTLMF AQSCTMIMNI ATRNEVMTGD QESSYKKAIQ QLRQEKRGKS
370 380 390 400 410 420
TANSSSTFEC IALKNQTLQL EIDASEKRYQ ELQKETSALR NRYQENLDAT ILMQTPSAKE
430 440 450 460 470 480
RRSSSRPKRR ETQYRPSPAR MALFAEVETS EDAVLDLQCQ NEKLCCELSS LKDKHEEATS
490 500 510 520 530 540
KSTETERLLQ GEIVALQKEV SIVSESLRKS EAVAEGYKEK VAANNLTLDK LLLHIDASRK
550 560 570 580 590 600
RIAELELSSK ESENRSSEEL EVDELQLSNA TIKKLNADLS TKAGLIRSLE DSIEKKSQMI
610 620 630 640 650 660
TRLEEQAVLD SSELSSTKRQ LEHSRLEAIQ WRKISDETTS AMQTQLKDHQ TDIDQCRHKK
670 680 690
KSEIQSMQLS LDNANARVKE SEAMMKKTSG
References and
documentation are available.
Number of amino acids: 690
Molecular weight: 77322.28
Theoretical pI: 7.00
Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 98
Atomic composition:
Carbon C 3310
Hydrogen H 5453
Nitrogen N 977
Oxygen O 1093
Sulfur S 28
Formula: C
3310H
5453N
977O
1093S
28
Total number of atoms: 10861
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36495
Abs 0.1% (=1 g/l) 0.472, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.464, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.94
This classifies the protein as unstable.
Aliphatic index: 81.57
Grand average of hydropathicity (GRAVY): -0.644