ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRAITRAQL DKLVRSITKF YPQKYADKSW DNTGLLIDCS TAQVTTADAN AKTKVLLTVD 

        70         80         90        100        110        120 
LTKSVAQEAV DANCNVIVAY HPFIFPSWNR LSPHTNPQHE TAIKLIQYGI SVYCPHTAVD 

       130        140        150        160        170        180 
AARGGVNDWL VRGLNNGENV AKSYALETVS GETDDLIGYG RFVEFNKDIS LEQIVKNVKR 

       190        200        210        220        230        240 
VLRVPYVQVA SLAAPSAWNQ LKIKKVAVCA GSGSGVFKQL KEDVDLYYTG EMSHHEVLKW 

       250        260        270        280 
KEMGKTVIVC NHSNTERGFL QDVMKGLLQD EGHEVVVSKM DCDPLTVA 

References and documentation are available.

Number of amino acids: 288

Molecular weight: 31888.44

Theoretical pI: 7.07

Amino acid composition: 
Ala (A)  24	  8.3%
Arg (R)  10	  3.5%
Asn (N)  15	  5.2%
Asp (D)  18	  6.2%
Cys (C)   6	  2.1%
Gln (Q)  12	  4.2%
Glu (E)  14	  4.9%
Gly (G)  17	  5.9%
His (H)   8	  2.8%
Ile (I)  13	  4.5%
Leu (L)  24	  8.3%
Lys (K)  22	  7.6%
Met (M)   5	  1.7%
Phe (F)   7	  2.4%
Pro (P)   9	  3.1%
Ser (S)  18	  6.2%
Thr (T)  18	  6.2%
Trp (W)   5	  1.7%
Tyr (Y)  10	  3.5%
Val (V)  33	 11.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1417
Hydrogen    H	      2248
Nitrogen    N	       388
Oxygen      O	       426
Sulfur      S	        11

Formula: C1417H2248N388O426S11
Total number of atoms: 4490

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42775
Abs 0.1% (=1 g/l)   1.341, assuming all pairs of Cys residues form cystines


Ext. coefficient    42400
Abs 0.1% (=1 g/l)   1.330, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.85
This classifies the protein as stable.



Aliphatic index: 91.67

Grand average of hydropathicity (GRAVY): -0.184