ProtParam
User-provided sequence:
10 20 30 40 50 60
MSRCIRQSVC TNFNVCRRQC FSTYASALKE MTHPIKPSAQ TLRHLQFTQR IPFQKGLEIQ
70 80 90 100 110 120
ETLVRANLDI KDIQSKIERK LIQLDEEYKG TATINDNEKR ILDKVMAMKP NPIILTFEFE
130 140 150 160 170 180
PTYTGGKRIK KTMTPDQIAA YESFIPETQK DNPRPKFVQV ERGGQVTFHG PGQIVIYIIL
190 200 210 220 230 240
DLKTFQSFPA KCLVSCIEQA TIRTLKNTKM CDDTDKPLNL DAMTTKDTGV WVENGKKKVA
250 260 270 280 290 300
SVGIHVRRSI TSHGVAINVN TDLSYMNSFE MCGLKNTLTT SIMEQRPDAV VNVQSVAISF
310 320
VKEMTKLLGI KTLERMQIDD VNILKKNP
References and
documentation are available.
Number of amino acids: 328
Molecular weight: 37237.30
Theoretical pI: 9.28
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 1640
Hydrogen H 2682
Nitrogen N 456
Oxygen O 488
Sulfur S 20
Formula: C
1640H
2682N
456O
488S
20
Total number of atoms: 5286
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14940
Abs 0.1% (=1 g/l) 0.401, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.388, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.30
This classifies the protein as stable.
Aliphatic index: 85.85
Grand average of hydropathicity (GRAVY): -0.349