ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRDLQNHLL FETATEVANR VGGIYSVLKS KAPITVAQYK DHYHLIGPLN KATYQNEVDI 

        70         80         90        100        110        120 
LDWKKPEAFS DEMRPVQHAL QTMESRGVHF VYGRWLIEGA PKVILFDLDS VRGYSNEWKG 

       130        140        150        160        170        180 
DLWSLVGIPS PENDFETNDA ILLGYTVAWF LGEVAHLDSQ HAIVAHFHEW LAGVALPLCR 

       190        200        210        220        230        240 
KRRIDVVTIF TTHATLLGRY LCASGSFDFY NCLESVDVDH EAGRFGIYHR YCIERAAAHS 

       250        260        270        280        290        300 
ADVFTTVSQI TAFEAEHLLK RKPDGILPNG LNVIKFQAFH EFQNLHALKK EKINDFVRGH 

       310        320        330        340        350        360 
FHGCFDFDLD NTLYFFIAGR YEYKNKGADM FIEALARLNY RLKVSGSKKT VVAFIVMPAK 

       370        380        390        400        410        420 
NNSFTVEALK GQAEVRALEN TVHEVTTSIG KRIFDHAIRY PHNGLTTELP TDLGELLKSS 

       430        440        450        460        470        480 
DKVMLKRRIL ALRRPEGQLP PIVTHNMVDD ANDLILNKIR QVQLFNSPSD RVKMIFHPEF 

       490        500        510        520        530        540 
LNANNPILGL DYDEFVRGCH LGVFPSYYEP WGYTPAECTV MGVPSITTNV SGFGAYMEDL 

       550        560        570        580        590        600 
IETNQAKDYG IYIVDRRFKA PDESVEQLVD YMEEFVKKTR RQRINQRNRT ERLSDLLDWK 

       610        620        630        640        650        660 
RMGLEYVKAR QLALRRGYPD QFRELVGEEL NDSNMDALAG GKKLKVARPL SVPGSPRDLR 

       670        680        690        700 
SNSTVYMTPG DLGTLQEVNN ADDYFSLGVN PAADDDDDGP YADDS 

References and documentation are available.

Number of amino acids: 705

Molecular weight: 80078.85

Theoretical pI: 5.91

Amino acid composition: 
Ala (A)  52	  7.4%
Arg (R)  44	  6.2%
Asn (N)  36	  5.1%
Asp (D)  52	  7.4%
Cys (C)   7	  1.0%
Gln (Q)  20	  2.8%
Glu (E)  44	  6.2%
Gly (G)  46	  6.5%
His (H)  24	  3.4%
Ile (I)  34	  4.8%
Leu (L)  70	  9.9%
Lys (K)  37	  5.2%
Met (M)  14	  2.0%
Phe (F)  36	  5.1%
Pro (P)  31	  4.4%
Ser (S)  35	  5.0%
Thr (T)  34	  4.8%
Trp (W)   8	  1.1%
Tyr (Y)  29	  4.1%
Val (V)  52	  7.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 96
Total number of positively charged residues (Arg + Lys): 81

Atomic composition:

Carbon      C	      3594
Hydrogen    H	      5552
Nitrogen    N	       986
Oxygen      O	      1052
Sulfur      S	        21

Formula: C3594H5552N986O1052S21
Total number of atoms: 11205

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    87585
Abs 0.1% (=1 g/l)   1.094, assuming all pairs of Cys residues form cystines


Ext. coefficient    87210
Abs 0.1% (=1 g/l)   1.089, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.54
This classifies the protein as stable.



Aliphatic index: 86.30

Grand average of hydropathicity (GRAVY): -0.340