ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSREDLSIAE DLNQVSKPLL KVKLLEVLGQ GDFKHLKALV DNEFQPKDDP SVQQVLNLIL 

        70         80         90        100        110        120 
HYAVQVAPIL LIKEIVAHWV DQVGDEKSSS KSDDGIHLDL NYQDENGNTP LHLAAAQSRS 

       130        140        150        160        170        180 
DVISFLLSQK SINDCVKNKA HQQPLDMCKD LNVAQMIQLK RDDYFLETVH SLRAAMNKRD 

       190        200        210        220        230        240 
FSKLDSIWKN PRNLNLLDIN GIDPETGTTL LYEYSQKKDI EMCQWLLKHG AEATVKDGKG 

       250        260        270        280        290        300 
RSPLDLVKNI KLPAKPSNNV TPEIKLKNLL EKNLREQAIV HEDVASSKPP TYKGFLKKWT 

       310        320        330        340        350        360 
NFAHGYKLRW FILSGDGNLS YYKDQSHVDR PRGTLKVSTC RLHIDSSEKL NFELLGGITG 

       370        380        390        400        410        420 
TTRWRLKGNH PIETTRWVNA IQSAIRFAKD KEILNKKKAV PPSLALKNKS PALISHSKTQ 

       430        440        450        460        470        480 
GSLPEASQYY QHTLHKEVIQ PSSVSLYRRP SNNLSVVSSE IQLNDNLTES GKRFVSKMIE 

       490        500        510        520        530        540 
NRLDGSKTPV GVHTGSALQR VRSSNTLKSN RSMQSGSGVA SPIDKVPNGA NLSQSNTTTG 

       550        560        570        580        590        600 
STASLSDNNY IDNFEGDEAN SDDEEEDLGI NFDRDEEYIK AQYGPYKEKL DMYEQAISIE 

       610        620        630        640        650        660 
LSSLIELIEQ EEPSPEVWLT IKKSLINTST IFGKLKDLTY KRDKRLVDMV SKQGDVNNVW 

       670        680        690        700        710        720 
VQSVKELEME LSNKTERLAS IDKERRGLKK ILHKKLLESH ATAGNKESLE NDKEQESDTT 

       730        740        750        760        770        780 
ASTLGQIAKF ISATKEEDEA SDADEFYDAA ELVDEVTELT EAHPEISTAA APKHAPPPVP 

       790        800        810        820        830        840 
NETDNDSQYV QDEKSKIESN VEKTSQKFEK QNNLVTEDEP KTDQSLKNFK AEDKESQVKE 

       850        860        870        880        890        900 
KTKEIASSVI GEKTIVAVTT VQKRKEEYLL KEGSYLGYED GIRKRLSMDK DDRPKISLWA 

       910        920        930        940        950        960 
VLKSMVGKDM TRMTLPVTFN EPTSLLQRVA EDLEYSELLD QAATFEDSTL RTLYVAAFTA 

       970        980        990       1000       1010       1020 
SSYASTTKRV AKPFNPLLGE TFEYSRPDKQ YRFFTEQVSH HPPISATWTE SPRWDFWGES 

      1030       1040       1050       1060       1070       1080 
FVDTKFNGRS FNVKHLGLWH IKLRPNDNEK EELYTWKKPN NTVIGILIGN PQVDNHGEVN 

      1090       1100       1110       1120       1130       1140 
VVNHTTGDHC KLYFKARGWR SSGAYEITGE VYNKKKQKVW ILGGHWNEAI FAKKVVKDGD 

      1150       1160       1170       1180       1190       1200 
LSLEKTRTAA SAGNGPTDDG TKFLIWKAND RPEEPFNLTP FAITLNAPQP HLLPWLPPTD 

      1210       1220       1230       1240       1250       1260 
TRLRPDQRAM EDGRYDEAGD EKFRVEEKQR AARRKREENN LEYHPQWFVR DTHPITKAKY 

      1270       1280 
WRYTGKYWVK RRDHDLKDCG DIF 

References and documentation are available.

Number of amino acids: 1283

Molecular weight: 145796.05

Theoretical pI: 6.37

Amino acid composition: 
Ala (A)  75	  5.8%
Arg (R)  59	  4.6%
Asn (N)  73	  5.7%
Asp (D)  90	  7.0%
Cys (C)   6	  0.5%
Gln (Q)  51	  4.0%
Glu (E) 103	  8.0%
Gly (G)  62	  4.8%
His (H)  33	  2.6%
Ile (I)  61	  4.8%
Leu (L) 122	  9.5%
Lys (K) 123	  9.6%
Met (M)  15	  1.2%
Phe (F)  37	  2.9%
Pro (P)  59	  4.6%
Ser (S) 103	  8.0%
Thr (T)  78	  6.1%
Trp (W)  23	  1.8%
Tyr (Y)  37	  2.9%
Val (V)  73	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 193
Total number of positively charged residues (Arg + Lys): 182

Atomic composition:

Carbon      C	      6452
Hydrogen    H	     10200
Nitrogen    N	      1796
Oxygen      O	      2012
Sulfur      S	        21

Formula: C6452H10200N1796O2012S21
Total number of atoms: 20481

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   182005
Abs 0.1% (=1 g/l)   1.248, assuming all pairs of Cys residues form cystines


Ext. coefficient   181630
Abs 0.1% (=1 g/l)   1.246, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.36
This classifies the protein as unstable.



Aliphatic index: 77.97

Grand average of hydropathicity (GRAVY): -0.747