ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSREGYPNFE EVEIPDFQET NNTVPDLDDL ELEYDQYKNN ENNDTFNDKD LESNSVAKHN 

        70         80         90        100        110        120 
AVNSSKGVKG SKIDYFNPSD VSLYDNSVSQ FEETTVSLKE YYDHSIRSHL TVKGACSYLK 

       130        140        150        160        170        180 
SVFPIINWLP HYNFSWFTAD LIAGITIGCV LVPQSMSYAQ VATLPAQYGL YSSFIGAYSY 

       190        200        210        220        230        240 
SFFATSKDVC IGPVAVMSLQ TAKVIADVTA KYPDGDSAIT GPVIATTLAL LCGIISAAVG 

       250        260        270        280        290        300 
FLRLGFLVEL ISLNAVAGFM TGSAFNILWG QVPALMGYNS LVNTRAATYK VVIETLKHLP 

       310        320        330        340        350        360 
DTKLDAVFGL IPLFLLYVWK WWCGTYGPRL NDRYNSKNPR LHKIIKWTYF YAQASRNGII 

       370        380        390        400        410        420 
IIVFTCIGWA ITRGKSKSER PISILGSVPS GLKEVGVFHV PPGLMSKLGP NLPASIIVLL 

       430        440        450        460        470        480 
LEHIAISKSF GRINDYKVVP DQELIAIGVS NLLGTFFNAY PATGSFSRSA LKAKCNVRTP 

       490        500        510        520        530        540 
LSGLFSGSCV LLALYCLTGA FFYIPKATLS AVIIHAVSDL LASYQTTWNF WKMNPLDFIC 

       550        560        570        580        590        600 
FIVTVLITVF ASIEDGIYFA MCWSCAMLIL KVAFPAGKFL GRVEVAEVTD AYVRPDSDVV 

       610        620        630        640        650        660 
SYVSENNNGI STLEDGGEDD KESSTKYVTN SSKKIETNVQ TKGFDSPSSS ISQPRIKYHT 

       670        680        690        700        710        720 
KWIPFDHKYT RELNPDVQIL PPPDGVLVYR LSESYTYLNC SRHYNIITEE VKKVTRRGQL 

       730        740        750        760        770        780 
IRHRKKSDRP WNDPGPWEAP AFLKNLKFWK KRENDPESME NAPSTSVDVE RDDRPLLKIL 

       790        800        810        820        830        840 
CLDFSQVAQT DATALQSLVD LRKAINQYAD RQVEFHFVGI ISPWVKRGLI SRGFGTLNEE 

       850        860        870        880        890 
YSDESIVAGH TSYHVARVPQ GEENPEKYSV YTASGTNLPF FHIDIPDFAK WDI 

References and documentation are available.

Number of amino acids: 893

Molecular weight: 99650.78

Theoretical pI: 6.23

Amino acid composition: 
Ala (A)  61	  6.8%
Arg (R)  32	  3.6%
Asn (N)  46	  5.2%
Asp (D)  49	  5.5%
Cys (C)  14	  1.6%
Gln (Q)  21	  2.4%
Glu (E)  42	  4.7%
Gly (G)  53	  5.9%
His (H)  17	  1.9%
Ile (I)  63	  7.1%
Leu (L)  79	  8.8%
Lys (K)  52	  5.8%
Met (M)  10	  1.1%
Phe (F)  45	  5.0%
Pro (P)  48	  5.4%
Ser (S)  81	  9.1%
Thr (T)  51	  5.7%
Trp (W)  17	  1.9%
Tyr (Y)  42	  4.7%
Val (V)  70	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 84

Atomic composition:

Carbon      C	      4541
Hydrogen    H	      6979
Nitrogen    N	      1159
Oxygen      O	      1317
Sulfur      S	        24

Formula: C4541H6979N1159O1317S24
Total number of atoms: 14020

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   156955
Abs 0.1% (=1 g/l)   1.575, assuming all pairs of Cys residues form cystines


Ext. coefficient   156080
Abs 0.1% (=1 g/l)   1.566, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.21
This classifies the protein as stable.



Aliphatic index: 91.58

Grand average of hydropathicity (GRAVY): -0.062