ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRFFSSNYE YDVASSSSEE DLLSSSEEDL LSSSSSESEL DQESDDSFFN ESESESEADV 

        70         80         90        100        110        120 
DSDDSDAKPY GPDWFKKSEF RKQGGGSNKF LKSSNYDSSD EESDEEDGKK VVKSAKEKLL 

       130        140        150        160        170        180 
DEMQDVYNKI SQAENSDDWL TISNEFDLIS RLLVRAQQQN WGTPNIFIKV VAQVEDAVNN 

       190        200        210        220        230        240 
TQQADLKNKA VARAYNTTKQ RVKKVSRENE DSMAKFRNDP ESFDKEPTAD LDISANGFTI 

       250        260        270        280        290        300 
SSSQGNDQAV QEDFFTRLQT IIDSRGKKTV NQQSLISTLE ELLTVAEKPY EFIMAYLTLI 

       310        320        330        340        350        360 
PSRFDASANL SYQPIDQWKS SFNDISKLLS ILDQTIDTYQ VNEFADPIDF IEDEPKEDSD 

       370        380        390        400        410        420 
GVKRILGSIF SFVERLDDEF MKSLLNIDPH SSDYLIRLRD EQSIYNLILR TQLYFEATLK 

       430        440        450        460        470        480 
DEHDLERALT RPFVKRLDHI YYKSENLIKI METAAWNIIP AQFKSKFTSK DQLDSADYVD 

       490        500        510        520        530        540 
NLIDGLSTIL SKQNNIAVQK RAILYNIYYT ALNKDFQTAK DMLLTSQVQT NINQFDSSLQ 

       550        560        570        580        590        600 
ILFNRVVVQL GLSAFKLCLI EECHQILNDL LSSSHLREIL GQQSLHRISL NSSNNASADE 

       610        620        630        640        650        660 
RARQCLPYHQ HINLDLIDVV FLTCSLLIEI PRMTAFYSGI KVKRIPYSPK SIRRSLEHYD 

       670        680        690        700        710        720 
KLSFQGPPET LRDYVLFAAK SMQKGNWRDS VKYLREIKSW ALLPNMETVL NSLTERVQVE 

       730        740        750        760        770        780 
SLKTYFFSFK RFYSSFSVAK LAELFDLPEN KVVEVLQSVI AELEIPAKLN DEKTIFVVEK 

       790        800        810 
GDEITKLEEA MVKLNKEYKI AKERLNPPSN RR 

References and documentation are available.

Number of amino acids: 812

Molecular weight: 93204.49

Theoretical pI: 4.86

Amino acid composition: 
Ala (A)  45	  5.5%
Arg (R)  37	  4.6%
Asn (N)  46	  5.7%
Asp (D)  67	  8.3%
Cys (C)   4	  0.5%
Gln (Q)  42	  5.2%
Glu (E)  66	  8.1%
Gly (G)  18	  2.2%
His (H)   9	  1.1%
Ile (I)  53	  6.5%
Leu (L)  85	 10.5%
Lys (K)  60	  7.4%
Met (M)  11	  1.4%
Phe (F)  41	  5.0%
Pro (P)  24	  3.0%
Ser (S)  94	 11.6%
Thr (T)  33	  4.1%
Trp (W)   7	  0.9%
Tyr (Y)  28	  3.4%
Val (V)  42	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 133
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      4136
Hydrogen    H	      6492
Nitrogen    N	      1096
Oxygen      O	      1322
Sulfur      S	        15

Formula: C4136H6492N1096O1322S15
Total number of atoms: 13061

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    80470
Abs 0.1% (=1 g/l)   0.863, assuming all pairs of Cys residues form cystines


Ext. coefficient    80220
Abs 0.1% (=1 g/l)   0.861, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.97
This classifies the protein as unstable.



Aliphatic index: 86.82

Grand average of hydropathicity (GRAVY): -0.523