ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRFKFIDIG INLTDPMFRG IYRGVQKHQD DLQDVIGRAV EIGVKKFMIT GGNLQDSKDA 

        70         80         90        100        110        120 
LHLAQTNGMF FSTVGCHPTR CGEFEKNNPD LYLKELLNLA ENNKGKVVAI GECGLDFDRL 

       130        140        150        160        170        180 
QFCPKDTQLK YFEKQFELSE QTKLPMFLHC RNSHAEFLDI MKRNRDRCVG GVVHSFDGTK 

       190        200        210        220        230        240 
EAAAALIDLD LYIGFNGCSL KTEANLEVLK SIPSEKLMIE TDAPWCGVKS THAGSKYIRT 

       250        260        270        280        290 
AFPTKKKWES GHCLKDRNEP CHIIQILEIM SAVRDEDPLE LANTLYNNTI KVFFPGI 

References and documentation are available.

Number of amino acids: 297

Molecular weight: 33601.66

Theoretical pI: 6.51

Amino acid composition: 
Ala (A)  16	  5.4%
Arg (R)  13	  4.4%
Asn (N)  16	  5.4%
Asp (D)  20	  6.7%
Cys (C)  10	  3.4%
Gln (Q)  10	  3.4%
Glu (E)  20	  6.7%
Gly (G)  22	  7.4%
His (H)   9	  3.0%
Ile (I)  20	  6.7%
Leu (L)  29	  9.8%
Lys (K)  25	  8.4%
Met (M)   8	  2.7%
Phe (F)  18	  6.1%
Pro (P)  11	  3.7%
Ser (S)  13	  4.4%
Thr (T)  15	  5.1%
Trp (W)   2	  0.7%
Tyr (Y)   6	  2.0%
Val (V)  14	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1494
Hydrogen    H	      2353
Nitrogen    N	       407
Oxygen      O	       438
Sulfur      S	        18

Formula: C1494H2353N407O438S18
Total number of atoms: 4710

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20565
Abs 0.1% (=1 g/l)   0.612, assuming all pairs of Cys residues form cystines


Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.593, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.62
This classifies the protein as stable.



Aliphatic index: 83.40

Grand average of hydropathicity (GRAVY): -0.318