ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRGRGGNYK DSQRFSLPFR KGQPQFYSST TTGQSRGPRV PSPSSLSGSS PHPSFYSSYS 

        70         80         90        100        110        120 
SGPGVPPRDH PFPPRHSDPS LWASPQHGSD QISDSNGVSF RQNQVQFLRG QSAQAPQFKA 

       130        140        150        160        170        180 
PQPRGSQSSS RGEEVSTEPI QYQTGYRDFK SNSYSFGRRN CYGRGQDNWQ GEQNFGPKKR 

       190        200        210        220        230        240 
SGWNHPHQKG QYYSNQHYKQ FWNARANDLS SGIYNLSFGD RVKRVDTSDT LSCSNSSSKV 

       250        260        270        280        290        300 
SSSSSSFRDS LYLTPEIHQQ VCAFLTSLRP AETIQAKVLG KKLGLPKKIV NKVLYDLLKS 

       310        320        330        340        350        360 
NQVVKQGETP PLWRLCEESR ESIREKDQTQ SSKDLEKEPG QSPYINQKVG GDFTTTSTPS 

       370        380        390        400        410        420 
PSKIPIDADS DTSDESEDSE GDDSCEKLLA DQDKKTLSPT VQSVTLSTMA DSKDSKEKIL 

       430        440        450        460        470        480 
QYLYEAGTGN ALVIAKNLGL RSAKQVNPTL YALEKQGDVN RNTEVNPPTW ELSAHRQEKM 

       490        500        510        520        530        540 
DRQRKAAATA NQDVNSAATL SLEDDEIANV ALVNFEPMEG GGKPLVEMLD NDNVFGNTEI 

       550        560        570        580        590        600 
DIPGLEPVSK ASDLSSSTHR NFQPNQFPYY QETPSNGGEQ SQWASDDIPE FLNTIRSEVA 

       610        620        630        640        650        660 
VSLAAPPPPT QSMESNRLQK LKEARSKNPV SGLMEFAQHL GYTCEFLLLE QSGPSHDPRF 

       670        680        690        700        710        720 
RMQVMLDGRR FPAAEGSNKK VAKKDAAAIT LKILWKELDG GGGEEEEEPH IEGAGSTTDM 

       730        740        750        760        770        780 
ADDMGGTDTP PQALSRSLPG GKNPVSVLME HSQRSGHPIQ FIKTGQEGPS HDPRFMFRVK 

       790        800        810        820        830        840 
VGERLFQEAS APSKKAARQL AAEEAVKELM GDGLLHLNKP PSSFCPMGDS ESQPMFPACP 

       850        860        870        880        890        900 
SLPPLTAAEL QAAHEAGVGD LINHLNNNAV SGLLELRPVL EASLLSIRLV GQSGPSHEPK 

       910        920        930        940        950        960 
FTYQAKLGGR WFPAVCASNK KQGKQEAADA ALRVLIGEAE KAARTGELMP ELPVSGSTIH 

       970        980        990       1000       1010       1020 
DHIAMLSHQR FNALTARIQH SLLGRKILAT IVMRNGTDSL GKVVSLGTGN RCVKGEELSL 

      1030       1040       1050       1060       1070       1080 
RGDTVNDCHA EIISRRGFIR FLYSELMKHW ESPGDETIFE LAGDGELKIK SDITFHLYIS 

      1090       1100       1110       1120       1130       1140 
TAPCGDGALF DKSCSEAAEL NGSGHMPLFE NIKQGKLRTK VENGEGTIPV ESSDIVPTWD 

      1150       1160       1170       1180       1190       1200 
GIQHGERLRT MSCSDKILRW NVLGLQGALL THFIHPIYLH SITLGYLYSH GHLTRAVCCR 

      1210       1220       1230       1240       1250       1260 
LSKDGDTFKS SLPANFTLNH PEVGRVSVYD STRHTSKTKE SSVNWSQPDQ YSVEVLDGTK 

      1270       1280       1290       1300       1310       1320 
GKLDSPKMEV SRVSKSNLFR LFHALCQRAG RADLLALQSY AHAKMAATSF QEAKRLFFLA 

      1330       1340       1350       1360       1370       1380 
LSQHGYGAWI GKPLEEKSFE GEAKSFEPMG GSGDVQASGC VVDPNSFLSS ENNNFLSAAQ 


GF 

References and documentation are available.

Number of amino acids: 1382

Molecular weight: 151492.29

Theoretical pI: 6.49

Amino acid composition: 
Ala (A)  95	  6.9%
Arg (R)  71	  5.1%
Asn (N)  59	  4.3%
Asp (D)  72	  5.2%
Cys (C)  18	  1.3%
Gln (Q)  75	  5.4%
Glu (E)  91	  6.6%
Gly (G) 113	  8.2%
His (H)  39	  2.8%
Ile (I)  46	  3.3%
Leu (L) 123	  8.9%
Lys (K)  82	  5.9%
Met (M)  25	  1.8%
Phe (F)  53	  3.8%
Pro (P)  87	  6.3%
Ser (S) 158	 11.4%
Thr (T)  65	  4.7%
Trp (W)  14	  1.0%
Tyr (Y)  31	  2.2%
Val (V)  65	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 163
Total number of positively charged residues (Arg + Lys): 153

Atomic composition:

Carbon      C	      6614
Hydrogen    H	     10385
Nitrogen    N	      1903
Oxygen      O	      2097
Sulfur      S	        43

Formula: C6614H10385N1903O2097S43
Total number of atoms: 21042

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   124315
Abs 0.1% (=1 g/l)   0.821, assuming all pairs of Cys residues form cystines


Ext. coefficient   123190
Abs 0.1% (=1 g/l)   0.813, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.58
This classifies the protein as unstable.



Aliphatic index: 68.21

Grand average of hydropathicity (GRAVY): -0.618