ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRHRAIRNL NLEDEMDDDY DDDYDDYEDE ENPEKQYTYD RNSLSTNYYT YLTDSGRNSP 

        70         80         90        100        110        120 
ALPAPHPAPT TSLSSTIVRN MTAPLLHQNQ KPPKNLNSRP STPQTSSNLN TPKRTPQVKN 

       130        140        150        160        170        180 
LQAESTTPTV SRPSSEVDLT SFRRNQLQNI AKAPSVARKT PKPRVADKDL INLIVVGHVD 

       190        200        210        220        230        240 
AGKSTLMGHL LHDLEVVDSR TIDKFKHEAA RNGKASFAYA WVLDETEEER ERGVTMDIGR 

       250        260        270        280        290        300 
TSFETSHRRI VLLDAPGHKD FISNMITGTS QADAAILVVN ATTGEFETGF ENGGQTKEHA 

       310        320        330        340        350        360 
LLLRSLGVTQ LIVAVNKLDT VDWSQDRFDE IKNNLSVFLT RQAGFSKPKF VPVSGFTGEN 

       370        380        390        400        410        420 
LIKRMELDWY DGPCLLELID SFVAPQPPSD GPLRIGISDV LKVASNQLVV SGKIESGEVE 

       430        440        450        460        470        480 
KDDKVYIMSS VTAATVKECA NNDGSRHCFA GDYILLTLQG TFEPESIQTG SVVVRAGPDT 

       490        500        510        520        530        540 
LIPSKKFEVR LVAFEIAMPI IKGAKAELYA HSLCVPCTFT NLLYTINKSN GEILKKGPRF 

       550        560        570        580        590 
IAKGASAVVE IETEYDIAIE TFTSCRALGR VTFRAGGNTI AAGIVEKVIT PQ 

References and documentation are available.

Number of amino acids: 592

Molecular weight: 65239.58

Theoretical pI: 5.61

Amino acid composition: 
Ala (A)  44	  7.4%
Arg (R)  32	  5.4%
Asn (N)  31	  5.2%
Asp (D)  39	  6.6%
Cys (C)   6	  1.0%
Gln (Q)  18	  3.0%
Glu (E)  39	  6.6%
Gly (G)  35	  5.9%
His (H)  12	  2.0%
Ile (I)  34	  5.7%
Leu (L)  50	  8.4%
Lys (K)  34	  5.7%
Met (M)   9	  1.5%
Phe (F)  21	  3.5%
Pro (P)  33	  5.6%
Ser (S)  46	  7.8%
Thr (T)  48	  8.1%
Trp (W)   3	  0.5%
Tyr (Y)  15	  2.5%
Val (V)  43	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2869
Hydrogen    H	      4574
Nitrogen    N	       798
Oxygen      O	       907
Sulfur      S	        15

Formula: C2869H4574N798O907S15
Total number of atoms: 9163

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39225
Abs 0.1% (=1 g/l)   0.601, assuming all pairs of Cys residues form cystines


Ext. coefficient    38850
Abs 0.1% (=1 g/l)   0.595, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.74
This classifies the protein as stable.



Aliphatic index: 83.83

Grand average of hydropathicity (GRAVY): -0.380