ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRKDDNQSG EVGRTLVDFM EVAITMIVYL KGFYPSAAFE RRRYMNVVVQ RARHPELRDY 

        70         80         90        100        110        120 
IHSAASGLLP FIEKGLVERV AVVFFSEDNV PVERFIFKIT IKPSCAALVE EGQLEFALRS 

       130        140        150        160        170        180 
FLIKLSVSKS LVKPLPLNCR WEVTAYLRSL PQVGSSKEAE LWIPTDTKQW QNPPVLTPVK 

       190        200        210 
SLNSEPLCLQ LYLEHPSLSE PLNLVNPEDV APHDP 

References and documentation are available.

Number of amino acids: 215

Molecular weight: 24393.24

Theoretical pI: 6.12

Amino acid composition: 
Ala (A)  13	  6.0%
Arg (R)  13	  6.0%
Asn (N)   8	  3.7%
Asp (D)   8	  3.7%
Cys (C)   3	  1.4%
Gln (Q)   7	  3.3%
Glu (E)  18	  8.4%
Gly (G)   7	  3.3%
His (H)   4	  1.9%
Ile (I)   9	  4.2%
Leu (L)  26	 12.1%
Lys (K)  11	  5.1%
Met (M)   4	  1.9%
Phe (F)  10	  4.7%
Pro (P)  18	  8.4%
Ser (S)  18	  8.4%
Thr (T)   7	  3.3%
Trp (W)   3	  1.4%
Tyr (Y)   6	  2.8%
Val (V)  22	 10.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      1108
Hydrogen    H	      1747
Nitrogen    N	       291
Oxygen      O	       314
Sulfur      S	         7

Formula: C1108H1747N291O314S7
Total number of atoms: 3467

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25565
Abs 0.1% (=1 g/l)   1.048, assuming all pairs of Cys residues form cystines


Ext. coefficient    25440
Abs 0.1% (=1 g/l)   1.043, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.65
This classifies the protein as unstable.



Aliphatic index: 99.21

Grand average of hydropathicity (GRAVY): -0.097