ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRKLKKTNL FNKDVSSLLY AYGDVPQPLQ ATVQCLDELV SGYLVDVCTN AFHTAQNSQR 

        70         80         90        100        110        120 
NKLRLEDFKF ALRKDPIKLG RAEELIATNK LITEAKKQFN ETDNQNSLKR YREEDEEGDE 

       130        140        150        160 
MEEDEDEQQV TDDDEEAAGR NSAKQSTDSK ATKIRKQGPK NLKKTKK 

References and documentation are available.

Number of amino acids: 167

Molecular weight: 19132.32

Theoretical pI: 5.66

Amino acid composition: 
Ala (A)  12	  7.2%
Arg (R)   9	  5.4%
Asn (N)  11	  6.6%
Asp (D)  15	  9.0%
Cys (C)   2	  1.2%
Gln (Q)  11	  6.6%
Glu (E)  17	 10.2%
Gly (G)   6	  3.6%
His (H)   1	  0.6%
Ile (I)   4	  2.4%
Leu (L)  16	  9.6%
Lys (K)  21	 12.6%
Met (M)   2	  1.2%
Phe (F)   5	  3.0%
Pro (P)   4	  2.4%
Ser (S)   9	  5.4%
Thr (T)  11	  6.6%
Trp (W)   0	  0.0%
Tyr (Y)   4	  2.4%
Val (V)   7	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	       821
Hydrogen    H	      1337
Nitrogen    N	       239
Oxygen      O	       278
Sulfur      S	         4

Formula: C821H1337N239O278S4
Total number of atoms: 2679

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     6085
Abs 0.1% (=1 g/l)   0.318, assuming all pairs of Cys residues form cystines


Ext. coefficient     5960
Abs 0.1% (=1 g/l)   0.312, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.06
This classifies the protein as unstable.



Aliphatic index: 66.05

Grand average of hydropathicity (GRAVY): -1.143