ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRKLSHLIA TRKSKTIVVT VLLIYSLLTF HLSNKRLLSQ FYPSKDDFKQ TLLPTTSHSQ 

        70         80         90        100        110        120 
DINLKKQITV NKKKNQLHNL RDQLSFAFPY DSQAPIPQRV WQTWKVGADD KNFPSSFRTY 

       130        140        150        160        170        180 
QKTWSGSYSP DYQYSLISDD SIIPFLENLY APVPIVIQAF KLMPGNILKA DFLRYLLLFA 

       190        200        210        220        230        240 
RGGIYSDMDT MLLKPIDSWP SQNKSWLNNI IDLNKPIPYK NSKPSLLSSD EISHQPGLVI 

       250        260        270        280        290        300 
GIEADPDRDD WSEWYARRIQ FCQWTIQAKP GHPILRELIL NITATTLASV QNPGVPVSEM 

       310        320        330        340        350        360 
IDPRFEEDYN VNYRHKRRHD ETYKHSELKN NKNVDGSDIM NWTGPGIFSD IIFEYMNNVL 

       370        380        390        400        410        420 
RYNSDILLIN PNLNKNDEEG SESATTPAKD VDNDTLSKST RKFYKKISES LQSSNSMPWE 

       430        440        450        460        470        480 
FFSFLKEPVI VDDVMVLPIT SFSPDVGQMG AQSSDDKMAF VKHMFSGSWK EDADKNAGHK 

References and documentation are available.

Number of amino acids: 480

Molecular weight: 55155.61

Theoretical pI: 7.19

Amino acid composition: 
Ala (A)  19	  4.0%
Arg (R)  18	  3.8%
Asn (N)  31	  6.5%
Asp (D)  38	  7.9%
Cys (C)   1	  0.2%
Gln (Q)  21	  4.4%
Glu (E)  18	  3.8%
Gly (G)  17	  3.5%
His (H)  11	  2.3%
Ile (I)  35	  7.3%
Leu (L)  44	  9.2%
Lys (K)  38	  7.9%
Met (M)  12	  2.5%
Phe (F)  22	  4.6%
Pro (P)  30	  6.2%
Ser (S)  51	 10.6%
Thr (T)  23	  4.8%
Trp (W)  11	  2.3%
Tyr (Y)  18	  3.8%
Val (V)  22	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2487
Hydrogen    H	      3843
Nitrogen    N	       657
Oxygen      O	       737
Sulfur      S	        13

Formula: C2487H3843N657O737S13
Total number of atoms: 7737

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    87320
Abs 0.1% (=1 g/l)   1.583, assuming all pairs of Cys residues form cystines


Ext. coefficient    87320
Abs 0.1% (=1 g/l)   1.583, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.99
This classifies the protein as unstable.



Aliphatic index: 81.44

Grand average of hydropathicity (GRAVY): -0.519