ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRKNSKKLK VYYLPVTLTQ FQKDLSEILI SLHAKSFKAS IIGEPQADAV NKPSGLPAGP 

        70         80         90        100        110        120 
ETHPYPTLSQ RQLTYIFDSN IRAIANHPSL LVDHYMPRQL LRMEPTESSI AGSHKFQVLN 

       130        140        150        160        170        180 
QLINSICFRD REGSPNEVIK CAIIAHSIKE LDLLEGLILG KKFRTKRLSG TSLYNEKHKF 

       190        200        210        220        230        240 
PNLPTVDSTI NKDGTPNSVS STSSNSNSTS YTGYSKDDYD YSVKRNLKKR KINTDDWLFL 

       250        260        270        280        290        300 
ATTKHLKHDQ YLLANYDIDM IISFDPMLEV ELPALQVLRN NANKDIPIIK LLVQNSPDHY 

       310        320        330        340        350        360 
LLDSEIKNSS VKSSHLSNNG HVDDSQEYEE IKSSLLYFLQ ARNAPVNNCE IDYIKLVKCC 

       370        380        390        400        410        420 
LEGKDCNNIL PVLDLITLDE ASKDSSDSGF WQPQLTKLQY SSTELPLWDG PLDIKTYQTE 

       430        440        450        460        470        480 
LMHRAVIRLR DIQDEYAKGT VPLYEKRLNE TQRQNQLDEI KNSVGLTFKK KQEVEKSIND 

       490        500        510        520        530        540 
SEKRLKHAMT ESTKLQNKIN HLLKNRQELE NFNKLPSNTI SSENHLEEGS ALADKLKEYI 

       550        560        570        580        590        600 
DKNATLFNKL KELQQANAEK SKLNDELRSK YQIESSKAAE SAQTLKILQE SMKSLENEVN 

       610        620        630        640        650        660 
GPLTKFSTES LKKELERLQN DFQSLKARNK FLKNYITLMN RQYDLKNKNN VQVEKAAANG 

       670 
TRFRSTRSNT PNYT 

References and documentation are available.

Number of amino acids: 674

Molecular weight: 77059.36

Theoretical pI: 8.81

Amino acid composition: 
Ala (A)  32	  4.7%
Arg (R)  27	  4.0%
Asn (N)  55	  8.2%
Asp (D)  39	  5.8%
Cys (C)   6	  0.9%
Gln (Q)  33	  4.9%
Glu (E)  47	  7.0%
Gly (G)  19	  2.8%
His (H)  16	  2.4%
Ile (I)  40	  5.9%
Leu (L)  83	 12.3%
Lys (K)  68	 10.1%
Met (M)   9	  1.3%
Phe (F)  18	  2.7%
Pro (P)  27	  4.0%
Ser (S)  66	  9.8%
Thr (T)  37	  5.5%
Trp (W)   3	  0.4%
Tyr (Y)  25	  3.7%
Val (V)  24	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 95

Atomic composition:

Carbon      C	      3398
Hydrogen    H	      5464
Nitrogen    N	       946
Oxygen      O	      1063
Sulfur      S	        15

Formula: C3398H5464N946O1063S15
Total number of atoms: 10886

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54125
Abs 0.1% (=1 g/l)   0.702, assuming all pairs of Cys residues form cystines


Ext. coefficient    53750
Abs 0.1% (=1 g/l)   0.698, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.66
This classifies the protein as unstable.



Aliphatic index: 86.25

Grand average of hydropathicity (GRAVY): -0.705