ProtParam
User-provided sequence:
10 20 30 40 50 60
MSRLERLTSL NVVAGSDLRR TSIIGTIGPK TNNPETLVAL RKAGLNIVRM NFSHGSYEYH
70 80 90 100 110 120
KSVIDNARKS EELYPGRPLA IALDTKGPEI RTGTTTNDVD YPIPPNHEMI FTTDDKYAKA
130 140 150 160 170 180
CDDKIMYVDY KNITKVISAG RIIYVDDGVL SFQVLEVVDD KTLKVKALNA GKICSHKGVN
190 200 210 220 230 240
LPGTDVDLPA LSEKDKEDLR FGVKNGVHMV FASFIRTAND VLTIREVLGE QGKDVKIIVK
250 260 270 280 290 300
IENQQGVNNF DEILKVTDGV MVARGDLGIE IPAPEVLAVQ KKLIAKSNLA GKPVICATQM
310 320 330 340 350 360
LESMTYNPRP TRAEVSDVGN AILDGADCVM LSGETAKGNY PINAVTTMAE TAVIAEQAIA
370 380 390 400 410 420
YLPNYDDMRN CTPKPTSTTE TVAASAVAAV FEQKAKAIIV LSTSGTTPRL VSKYRPNCPI
430 440 450 460 470 480
ILVTRCPRAA RFSHLYRGVF PFVFEKEPVS DWTDDVEARI NFGIEKAKEF GILKKGDTYV
490 500
SIQGFKAGAG HSNTLQVSTV
References and
documentation are available.
Number of amino acids: 500
Molecular weight: 54544.63
Theoretical pI: 7.56
Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 61
Atomic composition:
Carbon C 2414
Hydrogen H 3906
Nitrogen N 660
Oxygen O 737
Sulfur S 18
Formula: C
2414H
3906N
660O
737S
18
Total number of atoms: 7735
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28225
Abs 0.1% (=1 g/l) 0.517, assuming all pairs of Cys residues form cystines
Ext. coefficient 27850
Abs 0.1% (=1 g/l) 0.511, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 23.23
This classifies the protein as stable.
Aliphatic index: 92.60
Grand average of hydropathicity (GRAVY): -0.148