ProtParam
User-provided sequence:
10 20 30 40 50 60
MSRLLQTPFL PSVSLPTKTR SSVTGFRVKP RIIPIQAKIR EIFMPALSST MTEGKIVSWV
70 80 90 100 110 120
KSEGDKLNKG ESVVVVESDK ADMDVETFYD GYLAAIMVEE GGVAPVGSAI ALLAETEDEI
130 140 150 160 170 180
ADAKAKASGG GGGGDSKAPP ASPPTAAVEA PVSVEKKVAA APVSIKAVAA SAVHPASEGG
190 200 210 220 230 240
KRIVASPYAK KLAKELKVEL AGLVGSGPMG RIVAKDVEAV AAGGGVQAAV AVKEVVAAPG
250 260 270 280 290 300
VELGSVVPFT TMQGAVSRNM VESLGVPTFR VGYTISTDAL DALYKKIKSK GVTMTALLAK
310 320 330 340 350 360
ATALALAKHP VVNSSCRDGN SFVYNSSINV AVAVAIDGGL ITPVLQNADK VDIYSLSRKW
370 380 390 400 410 420
KELVDKARAK QLQPQEYNTG TFTLSNLGMF GVDRFDAILP PGTGAIMAVG ASQPSVVATK
430 440 450 460
DGRIGMKNQM QVNVTADHRV IYGADLAQFL QTLASIIEDP KDLTF
References and
documentation are available.
Number of amino acids: 465
Molecular weight: 48306.78
Theoretical pI: 8.80
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 50
Atomic composition:
Carbon C 2146
Hydrogen H 3509
Nitrogen N 577
Oxygen O 654
Sulfur S 14
Formula: C
2146H
3509N
577O
654S
14
Total number of atoms: 6900
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.505, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.505, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.14
This classifies the protein as stable.
Aliphatic index: 95.66
Grand average of hydropathicity (GRAVY): 0.131