ProtParam
User-provided sequence:
10 20 30 40 50 60
MSRMPSSFDV TERDLDDMTF GERIIYHCKK QPLVPIGCLL TTGAVILAAQ NVRLGNKWKA
70 80 90 100 110 120
QYYFRWRVGL QAATLVALVA GSFIYGTSGK ELKAKEEQLK EKAKMREKLW IQELERREEE
130 140 150
TEARRKRAEL ARMKTLENEE EIKNLEKELS DLENKLGKK
References and
documentation are available.
Number of amino acids: 159
Molecular weight: 18477.41
Theoretical pI: 9.16
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 816
Hydrogen H 1336
Nitrogen N 232
Oxygen O 241
Sulfur S 7
Formula: C
816H
1336N
232O
241S
7
Total number of atoms: 2632
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22585
Abs 0.1% (=1 g/l) 1.222, assuming all pairs of Cys residues form cystines
Ext. coefficient 22460
Abs 0.1% (=1 g/l) 1.216, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.19
This classifies the protein as unstable.
Aliphatic index: 84.72
Grand average of hydropathicity (GRAVY): -0.666