ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRMPSSFDV TERDLDDMTF GERIIYHCKK QPLVPIGCLL TTGAVILAAQ NVRLGNKWKA 

        70         80         90        100        110        120 
QYYFRWRVGL QAATLVALVA GSFIYGTSGK ELKAKEEQLK EKAKMREKLW IQELERREEE 

       130        140        150 
TEARRKRAEL ARMKTLENEE EIKNLEKELS DLENKLGKK 

References and documentation are available.

Number of amino acids: 159

Molecular weight: 18477.41

Theoretical pI: 9.16

Amino acid composition: 
Ala (A)  13	  8.2%
Arg (R)  13	  8.2%
Asn (N)   5	  3.1%
Asp (D)   5	  3.1%
Cys (C)   2	  1.3%
Gln (Q)   6	  3.8%
Glu (E)  21	 13.2%
Gly (G)   9	  5.7%
His (H)   1	  0.6%
Ile (I)   7	  4.4%
Leu (L)  19	 11.9%
Lys (K)  18	 11.3%
Met (M)   5	  3.1%
Phe (F)   4	  2.5%
Pro (P)   3	  1.9%
Ser (S)   6	  3.8%
Thr (T)   8	  5.0%
Trp (W)   3	  1.9%
Tyr (Y)   4	  2.5%
Val (V)   7	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	       816
Hydrogen    H	      1336
Nitrogen    N	       232
Oxygen      O	       241
Sulfur      S	         7

Formula: C816H1336N232O241S7
Total number of atoms: 2632

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22585
Abs 0.1% (=1 g/l)   1.222, assuming all pairs of Cys residues form cystines


Ext. coefficient    22460
Abs 0.1% (=1 g/l)   1.216, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.19
This classifies the protein as unstable.



Aliphatic index: 84.72

Grand average of hydropathicity (GRAVY): -0.666