ProtParam
User-provided sequence:
10 20 30 40 50 60
MSRPEHIAPP EIFYNDVEAG KYSTNTRIQS IQTEMSERAL ELLDAEGPSF ILDIGCGSGI
70 80 90 100 110 120
STQIGESQGH VVVGMDISPS MLSVALESQE IEGDLLLCDM GTGVPFRPGT FDGVISISAI
130 140 150 160 170 180
QWLLNADKTC NVPQRRLNRF FQTLYISMKR GGRAVMQYYP ETEKSQQMIM DTARKAGFAG
190 200 210 220 230 240
GIVVDHPESK RQKKYYLVLQ AGGTRTLDIS SMTLDQEGTN AKQRKLKKKQ DMSTREYIIH
250 260
KKELNRKRGR LHVPKDSKYS GRRRKAAF
References and
documentation are available.
Number of amino acids: 268
Molecular weight: 30083.44
Theoretical pI: 9.33
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1312
Hydrogen H 2123
Nitrogen N 381
Oxygen O 400
Sulfur S 14
Formula: C
1312H
2123N
381O
400S
14
Total number of atoms: 4230
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19035
Abs 0.1% (=1 g/l) 0.633, assuming all pairs of Cys residues form cystines
Ext. coefficient 18910
Abs 0.1% (=1 g/l) 0.629, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.53
This classifies the protein as unstable.
Aliphatic index: 76.42
Grand average of hydropathicity (GRAVY): -0.532