ProtParam
User-provided sequence:
10 20 30 40 50 60
MSRPIVISGP SGTGKSTLLK KLFAEYPDSF GFSVSSTTRT PRAGEVNGKD YNFVSVDEFK
70 80 90 100 110 120
SMIKNNEFIE WAQFSGNYYG STVASVKQVS KSGKTCILDI DMQGVKSVKA IPELNARFLF
130 140 150 160 170 180
IAPPSVEDLK KRLEGRGTET EESINKRLSA AQAELAYAET GAHDKVIVND DLDKAYKELK
DFIFAEK
References and
documentation are available.
Number of amino acids: 187
Molecular weight: 20637.37
Theoretical pI: 6.63
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 921
Hydrogen H 1454
Nitrogen N 242
Oxygen O 287
Sulfur S 4
Formula: C
921H
1454N
242O
287S
4
Total number of atoms: 2908
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.700, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.700, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 21.06
This classifies the protein as stable.
Aliphatic index: 76.15
Grand average of hydropathicity (GRAVY): -0.393