ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT 

        70         80         90        100        110        120 
SAESWGTGRA VARIPRVGGG GTGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY 

       130        140        150        160        170        180 
ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ KTKEAVAALK AVGAHSDLLK 

       190        200        210        220        230        240 
VLKSKKLRAG KGKYRNRRWT QRRGPLVVYA EDNGIVKALR NVPGVETANV ASLNLLQLAP 

       250        260        270        280        290        300 
GAHLGRFVIW TEAAFTKLDQ VWGSETVASS KVGYTLPSHI ISTSDVTRII NSSEIQSAIR 

       310        320        330        340        350        360 
PAGQATQKRT HVLKKNPLKN KQVLLRLNPY AKVFAAEKLG SKKAEKTGTK PAAVFTETLK 


HD 

References and documentation are available.

Number of amino acids: 362

Molecular weight: 39092.09

Theoretical pI: 10.64

Amino acid composition: 
Ala (A)  45	 12.4%
Arg (R)  25	  6.9%
Asn (N)  16	  4.4%
Asp (D)   7	  1.9%
Cys (C)   1	  0.3%
Gln (Q)  12	  3.3%
Glu (E)  16	  4.4%
Gly (G)  28	  7.7%
His (H)  10	  2.8%
Ile (I)  15	  4.1%
Leu (L)  28	  7.7%
Lys (K)  32	  8.8%
Met (M)   3	  0.8%
Phe (F)   8	  2.2%
Pro (P)  17	  4.7%
Ser (S)  25	  6.9%
Thr (T)  28	  7.7%
Trp (W)   6	  1.7%
Tyr (Y)   6	  1.7%
Val (V)  34	  9.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      1735
Hydrogen    H	      2841
Nitrogen    N	       523
Oxygen      O	       496
Sulfur      S	         4

Formula: C1735H2841N523O496S4
Total number of atoms: 5599

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41940
Abs 0.1% (=1 g/l)   1.073, assuming all pairs of Cys residues form cystines


Ext. coefficient    41940
Abs 0.1% (=1 g/l)   1.073, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.11
This classifies the protein as stable.



Aliphatic index: 85.99

Grand average of hydropathicity (GRAVY): -0.306