ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRRKISSES FSSLGSDYLE TSPEEEGECP LSKLCWNGSR SPPGPPGSRA AAMAATPVPA 

        70         80         90        100        110        120 
ASVAAAAAAV AAGSKRAQRR RRVNLDSLGE SISLLTAPSP QTIHMTLKRT LQYYEHQVIG 

       130        140        150        160        170        180 
YRDAEKNFHN ISNRCSSADH SNKEEIEDVS GILRCTANVL GLKFQEIQER FGEEFFKICF 

       190        200        210        220        230        240 
DENERVLRAV GSTLQDFFNG FDALLEHIRT SFGKQATLGS PSFLCKELPE GTLKLHYFHP 

       250        260        270        280        290        300 
HHTVGFAMLG MIKAAGKRIY HLNVEVEQIE NEKFCSDGST PSNYSCLTFL IKECETTQIT 

       310        320        330        340        350        360 
KNIPQGTSQI PTDLRISINT FCRTFPFHLM FDPNMVVLQL GEGLRKQLRC DNHKVLKFED 

       370        380        390        400        410        420 
CFEIVSPKVN ATFDRVLLRL STPFVIRTKP EASGTDNEDK VMEIKGQMIH VPESNAILFL 

       430        440        450        460        470        480 
GSPCVDKLDE LIGRGLHLSD IPIHDATRDV ILVGEQAKAQ DGLKKRMDKL KATLEKTHQA 

       490        500        510        520        530        540 
LEEEKKKTVD LLYSIFPGDV AQQLWQRQQV QARKFDDVTM LFSDIVGFTA ICAQCTPMQV 

       550        560        570        580        590        600 
ISMLNELYTR FDHQCGFLDI YKVETIGDAY CVASGLHRKS LCHAKPIALM ALKMMELSEE 

       610        620        630        640        650        660 
VLTPDGRPIQ MRIGIHSGSV LAGVVGVRMP RYCLFGNNVT LASKFESGSH PRRINISPTT 

       670        680        690        700        710        720 
YQLLKREDSF TFIPRSREEL PDNFPKEIPG VCYFLELRTG PKPPKPSLSS SRIKKVSYNI 

       730        740 
GTMFLRETSL VPRGSWSHPQ FEK 

References and documentation are available.

Number of amino acids: 743

Molecular weight: 83251.60

Theoretical pI: 8.14

Amino acid composition: 
Ala (A)  45	  6.1%
Arg (R)  45	  6.1%
Asn (N)  25	  3.4%
Asp (D)  34	  4.6%
Cys (C)  20	  2.7%
Gln (Q)  30	  4.0%
Glu (E)  53	  7.1%
Gly (G)  46	  6.2%
His (H)  22	  3.0%
Ile (I)  44	  5.9%
Leu (L)  71	  9.6%
Lys (K)  46	  6.2%
Met (M)  19	  2.6%
Phe (F)  39	  5.2%
Pro (P)  42	  5.7%
Ser (S)  61	  8.2%
Thr (T)  42	  5.7%
Trp (W)   3	  0.4%
Tyr (Y)  15	  2.0%
Val (V)  41	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 87
Total number of positively charged residues (Arg + Lys): 91

Atomic composition:

Carbon      C	      3686
Hydrogen    H	      5856
Nitrogen    N	      1026
Oxygen      O	      1091
Sulfur      S	        39

Formula: C3686H5856N1026O1091S39
Total number of atoms: 11698

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40100
Abs 0.1% (=1 g/l)   0.482, assuming all pairs of Cys residues form cystines


Ext. coefficient    38850
Abs 0.1% (=1 g/l)   0.467, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.04
This classifies the protein as unstable.



Aliphatic index: 82.42

Grand average of hydropathicity (GRAVY): -0.295