ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK 

        70         80         90        100        110        120 
KDDPEYAEER EKMLLQIGVE ADAGRSNVKV KDEDPNEYNE FPLRAIPKED LENMRTHLLK 

       130        140        150        160        170        180 
FQSKKKINPV TDFHLPVRLH RKDTRNLQFQ LTRAEIVQRQ KEISEYKKKA EQERSTPNSG 

       190        200        210        220        230        240 
GMNKSGTVSL NNTVKDGSQT PTVDSVTKDN TANGVNSSIP TVTGSSVPPA SPTTVSAVES 

       250        260        270        280        290        300 
NGLSNGSTSA ANGLDGNAST ANLANGRPLV TKLEDAGPAE DPTKVGMVKY DGKEVTNEPE 

       310        320        330        340        350        360 
FEEGTMDPLA DVAPDGGGRA KRGNLRRKTR QLKVLDENAK KLRFEEFYPW VMEDFDGYNT 

       370        380        390        400        410        420 
WVGSYEAGNS DSYVLLSVED DGSFTMIPAD KVYKFTARNK YATLTIDEAE KRMDKKSGEV 

       430        440        450        460        470        480 
PRWLMKHLDN IGTTTTRYDR TRRKLKAVAD QQAMDEDDRD DNSEVELDYD EEFADDEEAP 

       490        500        510        520        530        540 
IIDGNEQENK ESEQRIKKEM LQANAMGLRD EEAPSENEED ELFGEKKIDE DGERIKKALQ 

       550        560        570        580        590        600 
KTELAALYSS DENEINPYLS ESDIENKENE SPVKKEEDSD TLSKSKRSSP KKQQKKATNA 

       610        620        630        640        650        660 
HVHKEPTLRV KSIKNCVIIL KGDKKILKSF PEGEWNPQTT KAVDSSNNAS NTVPSPIKQE 

       670        680        690        700        710        720 
EGLNSTVAER EETPAPTITE KDIIEAIGDG KVNIKEFGKF IRRKYPGAEN KKLMFAIVKK 

       730 
LCRKVGNDHM ELKKE 

References and documentation are available.

Number of amino acids: 735

Molecular weight: 82194.43

Theoretical pI: 5.57

Amino acid composition: 
Ala (A)  46	  6.3%
Arg (R)  44	  6.0%
Asn (N)  53	  7.2%
Asp (D)  55	  7.5%
Cys (C)   2	  0.3%
Gln (Q)  20	  2.7%
Glu (E)  76	 10.3%
Gly (G)  48	  6.5%
His (H)   7	  1.0%
Ile (I)  29	  3.9%
Leu (L)  45	  6.1%
Lys (K)  75	 10.2%
Met (M)  18	  2.4%
Phe (F)  18	  2.4%
Pro (P)  40	  5.4%
Ser (S)  55	  7.5%
Thr (T)  44	  6.0%
Trp (W)   4	  0.5%
Tyr (Y)  15	  2.0%
Val (V)  41	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 131
Total number of positively charged residues (Arg + Lys): 119

Atomic composition:

Carbon      C	      3529
Hydrogen    H	      5693
Nitrogen    N	      1033
Oxygen      O	      1185
Sulfur      S	        20

Formula: C3529H5693N1033O1185S20
Total number of atoms: 11460

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44475
Abs 0.1% (=1 g/l)   0.541, assuming all pairs of Cys residues form cystines


Ext. coefficient    44350
Abs 0.1% (=1 g/l)   0.540, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.78
This classifies the protein as unstable.



Aliphatic index: 61.70

Grand average of hydropathicity (GRAVY): -1.037