ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRSGVAVAD ESLTAFNDLK LGKKYKFILF GLNDAKTEIV VKETSTDPSY DAFLEKLPEN 

        70         80         90        100        110        120 
DCLYAIYDFE YEINGNEGKR SKIVFFTWSP DTAPVRSKMV YASSKDALRR ALNGVSTDVQ 

       130        140 
GTDFSEVSYD SVLERVSRGA GSH 

References and documentation are available.

Number of amino acids: 143

Molecular weight: 15900.77

Theoretical pI: 5.05

Amino acid composition: 
Ala (A)  11	  7.7%
Arg (R)   7	  4.9%
Asn (N)   6	  4.2%
Asp (D)  12	  8.4%
Cys (C)   1	  0.7%
Gln (Q)   1	  0.7%
Glu (E)  10	  7.0%
Gly (G)   9	  6.3%
His (H)   1	  0.7%
Ile (I)   5	  3.5%
Leu (L)  11	  7.7%
Lys (K)  11	  7.7%
Met (M)   2	  1.4%
Phe (F)   8	  5.6%
Pro (P)   4	  2.8%
Ser (S)  16	 11.2%
Thr (T)   8	  5.6%
Trp (W)   1	  0.7%
Tyr (Y)   7	  4.9%
Val (V)  12	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 18

Atomic composition:

Carbon      C	       707
Hydrogen    H	      1097
Nitrogen    N	       185
Oxygen      O	       226
Sulfur      S	         3

Formula: C707H1097N185O226S3
Total number of atoms: 2218

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15930
Abs 0.1% (=1 g/l)   1.002, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   1.002, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.14
This classifies the protein as unstable.



Aliphatic index: 75.66

Grand average of hydropathicity (GRAVY): -0.380