ProtParam
User-provided sequence:
10 20 30 40 50 60
MSRSLLVENS RTINSNEEKG VNESQYILQK RNVPRTILGN VTNNANILQE ISMNRKIGMK
70 80 90 100 110 120
NFSKLNNFFP LKDDVSRADD FTSSFNDSRQ GVKQEVLNNK ENIPEYGYSE QEKQQCSNDD
130 140 150 160 170 180
SFHTNSTALS CNRLIYSENK SISTQMEWQK KIMREDSKKK RPISTLVEQD DQKKFKLHEL
190 200 210 220 230 240
TTEEEVLEEY EWDDLDEEDC DDPLMVSEEV NDIFDYLHHL EIITLPNKAN LYKHKNIKQN
250 260 270 280 290 300
RDILVNWIIK IHNKFGLLPE TLYLAINIMD RFLCEEVVQL NRLQLVGTSC LFIASKYEEI
310 320 330 340 350 360
YSPSIKHFAY ETDGACSVED IKEGERFILE KLDFQISFAN PMNFLRRISK ADDYDIQSRT
370 380 390 400 410 420
LAKFLMEISI VDFKFIGILP SLCASAAMFL SRKMLGKGTW DGNLIHYSGG YTKAKLYPVC
430 440 450 460 470
QLLMDYLVGS TIHDEFLKKY QSRRFLKASI ISIEWALKVR KNGYDIMTLH E
References and
documentation are available.
Number of amino acids: 471
Molecular weight: 54870.51
Theoretical pI: 5.64
Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 61
Atomic composition:
Carbon C 2440
Hydrogen H 3840
Nitrogen N 652
Oxygen O 743
Sulfur S 21
Formula: C
2440H
3840N
652O
743S
21
Total number of atoms: 7696
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 54820
Abs 0.1% (=1 g/l) 0.999, assuming all pairs of Cys residues form cystines
Ext. coefficient 54320
Abs 0.1% (=1 g/l) 0.990, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.03
This classifies the protein as unstable.
Aliphatic index: 87.96
Grand average of hydropathicity (GRAVY): -0.498