ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRTNMDTRH AHSALLAAPQ SATANSRSSN SSSESSSNKN NINVGVGDDS GNVSAVSIDD 

        70         80         90        100        110        120 
GPHFRDIFHY GHEENYKLAS SGITNLNSSS HAHQTLSPIS ISNASTPESF PEHPLGLERE 

       130        140        150        160        170        180 
TEPALEAEME AEELPPHQSK YLLSSIKATK RLKDARPFLK PVDPIALNIP HYFNYVQTPM 

       190        200        210        220        230        240 
DLSLIETKLQ GNVYHSVEQV TSDFKTMVDN CLNFNGPESS ISSMAKRIQK YFEKKLSAMP 

       250        260        270        280        290        300 
PRVLPASALK KTSRNRKKNE DMDSPLVIRR SVSTTNDNIG ESGNREGVSG GRPKRTIHPP 

       310        320        330        340        350        360 
KSKDLFDIYE NSKPKSKTLQ KKFRTCLKIL KVLMSKKNSD INFPFLQPVD PIALNLPNYF 

       370        380        390        400        410        420 
DVVKNPMDLG TISNNLMNWK YKTIDQFVDD LNLVFYNCFQ FNPEGNEVHS MGKKLKELFN 

       430        440        450        460        470        480 
FHWLENQDIL NEIETDSDLE EDNYSSSYSS DDEYDDEDIN ENDITNPAIQ YLEQKLKKME 

       490        500        510        520        530        540 
VELQQLKRQE LSKLSKERKR KHLGKTLLRR KAMKHSVDDL KKSITDKINE LSDLEMNGMI 

       550        560        570        580        590        600 
RIIKNSLPAD EILTSNEDEI EIDLDILDEA TIARIYERYF EKKNNNNSKR KLSGNYSTAP 

       610        620        630 
TNKKKKTLKF LEKDEIINNN NYSDSEEDSS DSSDSDSD 

References and documentation are available.

Number of amino acids: 638

Molecular weight: 72513.07

Theoretical pI: 5.77

Amino acid composition: 
Ala (A)  27	  4.2%
Arg (R)  26	  4.1%
Asn (N)  57	  8.9%
Asp (D)  49	  7.7%
Cys (C)   3	  0.5%
Gln (Q)  17	  2.7%
Glu (E)  50	  7.8%
Gly (G)  20	  3.1%
His (H)  16	  2.5%
Ile (I)  39	  6.1%
Leu (L)  58	  9.1%
Lys (K)  60	  9.4%
Met (M)  16	  2.5%
Phe (F)  21	  3.3%
Pro (P)  32	  5.0%
Ser (S)  74	 11.6%
Thr (T)  29	  4.5%
Trp (W)   2	  0.3%
Tyr (Y)  19	  3.0%
Val (V)  23	  3.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      3158
Hydrogen    H	      5024
Nitrogen    N	       884
Oxygen      O	      1033
Sulfur      S	        19

Formula: C3158H5024N884O1033S19
Total number of atoms: 10118

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39435
Abs 0.1% (=1 g/l)   0.544, assuming all pairs of Cys residues form cystines


Ext. coefficient    39310
Abs 0.1% (=1 g/l)   0.542, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.19
This classifies the protein as unstable.



Aliphatic index: 73.98

Grand average of hydropathicity (GRAVY): -0.839