ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRYDSRTGD STSYRDRRSD SGFGGTSSYG SSGSHTSSKK DNDGNESPRK LDLDGLTPFE 

        70         80         90        100        110        120 
KNFYVESPAV AAMTDTEVEE YRKLREITVE GKDIPKPVKS FRDVGFPDYV LEEVKKAGFT 

       130        140        150        160        170        180 
EPTPIQSQGW PMAMKGRDLI GIAETGSGKT LSYLLPAIVH VNAQPMLAHG DGPIVLVLAP 

       190        200        210        220        230        240 
TRELAVQIQQ EASKFGSSSK IKTTCIYGGV PKGPQVRDLQ KGVEIVIATP GRLIDMMESN 

       250        260        270        280        290        300 
NTNLRRVTYL VLDEADRMLD MGFDPQIRKI VSHIRPDRQT LYWSATWPKE VEQLSKKFLY 

       310        320        330        340        350        360 
NPYKVIIGSS DLKANRAIRQ IVDVISESQK YNKLVKLLED IMDGSRILVF LDTKKGCDQI 

       370        380        390        400        410        420 
TRQLRMDGWP ALSIHGDKSQ AERDWVLSEF RSGKSPIMTA TDVAARGLDV KDVKYVINYD 

       430        440        450        460        470        480 
FPGSLEDYVH RIGRTGRAGA KGTAYTFFTV ANARFAKELT NILQEAGQKV SPELASMGRS 

       490        500 
TAPPPPGLGG FRDRGSRRGW S 

References and documentation are available.

Number of amino acids: 501

Molecular weight: 55576.13

Theoretical pI: 9.10

Amino acid composition: 
Ala (A)  31	  6.2%
Arg (R)  36	  7.2%
Asn (N)  13	  2.6%
Asp (D)  36	  7.2%
Cys (C)   2	  0.4%
Gln (Q)  18	  3.6%
Glu (E)  27	  5.4%
Gly (G)  44	  8.8%
His (H)   6	  1.2%
Ile (I)  29	  5.8%
Leu (L)  37	  7.4%
Lys (K)  35	  7.0%
Met (M)  13	  2.6%
Phe (F)  16	  3.2%
Pro (P)  28	  5.6%
Ser (S)  43	  8.6%
Thr (T)  30	  6.0%
Trp (W)   6	  1.2%
Tyr (Y)  17	  3.4%
Val (V)  34	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 71

Atomic composition:

Carbon      C	      2453
Hydrogen    H	      3911
Nitrogen    N	       693
Oxygen      O	       749
Sulfur      S	        15

Formula: C2453H3911N693O749S15
Total number of atoms: 7821

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58455
Abs 0.1% (=1 g/l)   1.052, assuming all pairs of Cys residues form cystines


Ext. coefficient    58330
Abs 0.1% (=1 g/l)   1.050, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.07
This classifies the protein as unstable.



Aliphatic index: 77.25

Grand average of hydropathicity (GRAVY): -0.495