ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSRYQSHSFD DGEINPFANP TSVPAATSKL SPLPPEPYDR GATMDIPLDS GKDLKAKEKE 

        70         80         90        100        110        120 
LREKEAELKR REQEIKRKED AIAQAGIVIE EKNWPPFFPL IHHDISNEIP IHLQRIQYVA 

       130        140        150        160        170        180 
FTSMLGLVVC LLWNIVAVTT AWIKGEGPTI WFLAIIYFIS GVPGAYVMWY RPLYRAMRTD 

       190        200        210        220        230        240 
SALKFGWFFF TYLFHIAFCV FAAVAPPIIF KGKSLTGILP AIDVLSGNIL VGIFYFIGFG 

       250        260        270        280 
FFCLESLVSI WVIQQVYMYF RGSGKAAEMK QEATRRAMMA AL 

References and documentation are available.

Number of amino acids: 282

Molecular weight: 31963.41

Theoretical pI: 8.32

Amino acid composition: 
Ala (A)  27	  9.6%
Arg (R)  13	  4.6%
Asn (N)   6	  2.1%
Asp (D)  10	  3.5%
Cys (C)   3	  1.1%
Gln (Q)   8	  2.8%
Glu (E)  17	  6.0%
Gly (G)  18	  6.4%
His (H)   5	  1.8%
Ile (I)  27	  9.6%
Leu (L)  23	  8.2%
Lys (K)  16	  5.7%
Met (M)   9	  3.2%
Phe (F)  21	  7.4%
Pro (P)  18	  6.4%
Ser (S)  16	  5.7%
Thr (T)  11	  3.9%
Trp (W)   7	  2.5%
Tyr (Y)  11	  3.9%
Val (V)  16	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1491
Hydrogen    H	      2274
Nitrogen    N	       368
Oxygen      O	       389
Sulfur      S	        12

Formula: C1491H2274N368O389S12
Total number of atoms: 4534

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55015
Abs 0.1% (=1 g/l)   1.721, assuming all pairs of Cys residues form cystines


Ext. coefficient    54890
Abs 0.1% (=1 g/l)   1.717, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.18
This classifies the protein as unstable.



Aliphatic index: 95.18

Grand average of hydropathicity (GRAVY): 0.179