ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSAFVVGAT GAVGSELVKL LAESTKFSKV VVLARRPVDG ATGDKLIQKT VDFDKLEENA 

        70         80         90        100        110        120 
EDIQGVDVAF CALGTTRGKS GADGFYKVDH DYVMSAAKMA KENGVKQFVL VSSVGADASS 

       130        140        150        160        170        180 
RFLYPKTKGE VEKEIGELNF EKFVIMRPGL IEAKRPEFRI GEFLGKIVTA PLGLFSNRFS 

       190        200        210 
SSATAIAQAM INATQTEETG NQIWNNSKIV EESKKYTA 

References and documentation are available.

Number of amino acids: 218

Molecular weight: 23545.88

Theoretical pI: 6.64

Amino acid composition: 
Ala (A)  23	 10.6%
Arg (R)   8	  3.7%
Asn (N)   8	  3.7%
Asp (D)  10	  4.6%
Cys (C)   1	  0.5%
Gln (Q)   6	  2.8%
Glu (E)  18	  8.3%
Gly (G)  19	  8.7%
His (H)   1	  0.5%
Ile (I)  11	  5.0%
Leu (L)  14	  6.4%
Lys (K)  20	  9.2%
Met (M)   5	  2.3%
Phe (F)  13	  6.0%
Pro (P)   5	  2.3%
Ser (S)  17	  7.8%
Thr (T)  13	  6.0%
Trp (W)   1	  0.5%
Tyr (Y)   4	  1.8%
Val (V)  21	  9.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1048
Hydrogen    H	      1677
Nitrogen    N	       279
Oxygen      O	       323
Sulfur      S	         6

Formula: C1048H1677N279O323S6
Total number of atoms: 3333

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.487, assuming all pairs of Cys residues form cystines


Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.487, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.14
This classifies the protein as stable.



Aliphatic index: 83.21

Grand average of hydropathicity (GRAVY): -0.128