ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSDAIRNTE QINAAIKIIE NKTERPQSST TPIDSKASTV AAANSTATET SRDLTQYTLD
70 80 90 100 110 120
DGRVVSTNRR IMNKVPAITS HVPTDEELFQ PNGIPRHEFL RDHFKREGKL SAAQAARIVT
130 140 150 160 170 180
LATELFSKEP NLISVPAPIT VCGDIHGQYF DLLKLFEVGG DPATTSYLFL GDYVDRGSFS
190 200 210 220 230 240
FECLIYLYSL KLNFNDHFWL LRGNHECKHL TSYFTFKNEM LHKYNLDIYE KCCESFNNLP
250 260 270 280 290 300
LAALMNGQYL CVHGGISPEL NSLQDINNLN RFREIPSHGL MCDLLWADPI EEYDEVLDKD
310 320 330 340 350 360
LTEEDIVNSK TMVPHHGKMA PSRDMFVPNS VRGCSYAFTY RAACHFLQET GLLSIIRAHE
370 380 390 400 410 420
AQDAGYRMYK NTKTLGFPSL LTLFSAPNYL DTYNNKAAIL KYENNVMNIR QFNMTPHPYW
430 440 450 460 470 480
LPDFMDVFTW SLPFVGEKVT EMLVAILNIC TEDELENDTP VIEELVGTDK KLPQAGKSEA
490 500 510 520 530 540
TPQPATSASP KHASILDDEH RRKALRNKIL AVAKVSRMYS VLREETNKVQ FLKDHNSGVL
550 560 570 580 590 600
PRGALSNGVK GLDEALSTFE RARKHDLINE KLPPSLDELK NENKKYYEKV WQKVHEHDAK
NDSK
References and
documentation are available.
Number of amino acids: 604
Molecular weight: 68527.69
Theoretical pI: 5.92
Total number of negatively charged residues (Asp + Glu): 81
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 3045
Hydrogen H 4754
Nitrogen N 830
Oxygen O 923
Sulfur S 24
Formula: C
3045H
4754N
830O
923S
24
Total number of atoms: 9576
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 60905
Abs 0.1% (=1 g/l) 0.889, assuming all pairs of Cys residues form cystines
Ext. coefficient 60280
Abs 0.1% (=1 g/l) 0.880, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.67
This classifies the protein as unstable.
Aliphatic index: 81.89
Grand average of hydropathicity (GRAVY): -0.452