ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSDASTYRL EDVLSSFYRV EKIKKINYHQ YISKAQNDQW SIQMEFMLRK QDPKTLVALL 

        70         80         90        100        110        120 
SRDLWCFSIN DDPVPTPPAI EHKPVSPDKI GTFTADYSKP NLPPHYALFL KALRRKIYIN 

       130        140        150        160        170        180 
LALGSHNKLI QFGNACISLS GVPNYLVQLE PHLFVNGDLT VSLCAKNMGL VPMKEENLEE 

       190        200        210        220        230        240 
SFLSKHALYL APSGIRMHLA PASKQGYLIT PPKHTELLLT TLSVSHGINL QNKKNLKWVA 

       250        260        270        280        290        300 
VVPDLGHLNG HTPTIASYLT PLLEAKKLVW PLHLIFAQPV ADIENSTSGD PSEFHCLQDA 

       310        320        330        340        350        360 
LDAIDDFIQL KQTAAYRTPG SSGVLSSNIA GTNPLSSDGA YTEQFQHYKN NSISSQPASY 

       370        380        390        400        410        420 
HSVQETNKIS PKDFSPNFTG IDKLMLSPSD QFAPAFLNTP NNNINENELF NDRKQTTVSN 

       430        440        450        460        470        480 
DLENSPLKTE LEANGRSLEK VNNSVSKTGS VDTLHNKEGT LEQREQNENL PSDKSDSMVD 

       490        500        510        520        530        540 
KELFGEDEDE DLFGDSNKSN STNESNKSIS DEITEDMFEM SDEEENNNNK SINKNNKEMH 

       550        560        570        580        590        600 
TDLGKDIPFF PSSEKPNIRT MSGTTKRLNG KRKYLDIPID EMTLPTSPLY MDPGAPLPVE 

       610        620        630        640        650        660 
TPRDRRKSVF APLNFNPIIE NNVDNKYKSG GKFSFSPLQK EEALNFDISM ADLSSSEEEE 

       670        680        690        700        710        720 
DEEENGSSDE DLKSLNVRDD MKPSDNISTN TNIHEPQYIN YSSIPSLQDS IIKQENFNSV 

       730        740        750        760        770        780 
NDANITSNKE GFNSIWKIPQ NDIPQTESPL KTVDSSIQPI ESNIKMTLED NNVTSNPSEF 

       790        800        810        820        830        840 
TPNMVNSEIS NLPKDKSGIP EFTPADPNLS FESSSSLPFL LRHMPLASIP DIFITPTPVV 

       850        860        870        880        890        900 
TISEKEQDIL DLIAEQVVTD YNILGNLGIP KIAYRGVKDC QEGLITTTML QLFSTFDRLN 

       910        920        930        940        950        960 
GNDTISKFYN MKQPYVFVKK HHELIKVKHD SQPFIKFLNF RPPNGIKNFK SLLLSSSFKE 

       970        980        990       1000       1010       1020 
DCLSFAPTLS QTYINQELGF CELLKLTNED PPGLMYLKAF DKNKLLLLAA QIVSYCSNNK 

      1030       1040       1050       1060       1070       1080 
NSIKNVPPIL IILPLDNATL TELVDKANIF QVIKNEVCAK MPNIELYLKV IPMDFIRNVL 

      1090       1100       1110       1120       1130       1140 
VTVDQYVNVA ISIYNMLPPK SVKFTHIAHT LPEKVNFRTM QQQQMQQQQQ QQQQQQNNST 

      1150       1160       1170       1180       1190       1200 
GSSSIIYYDS YIHLAYSRSV DKEWVFAALS DSYGQGSMTK TWYVGNSRGK FDDACNQIWN 

      1210       1220       1230       1240       1250       1260 
IALNLASKKF GKICLILTRL NGILPDDELM NWRRLSGRNI HLAVVCVDDN SKISFIDEDK 

      1270       1280       1290       1300       1310       1320 
LYPSFKPIYK DTRFGGRMDM TRLYDYEIRD IDQDIHGIVF QHPFPLAHSQ HRCAIRSGAL 

      1330       1340       1350       1360       1370       1380 
IKFKKCDGDT VWDKFAVNLL NCPHSDSTQL LETILEEFRN LAALNVWYGL SDGEDGHIPW 

      1390       1400       1410       1420 
HILAVKKMMN TLVHTRVKIA NTSAATVHTA TSSSIILSDK 

References and documentation are available.

Number of amino acids: 1420

Molecular weight: 160000.96

Theoretical pI: 5.48

Amino acid composition: 
Ala (A)  65	  4.6%
Arg (R)  38	  2.7%
Asn (N) 116	  8.2%
Asp (D)  95	  6.7%
Cys (C)  15	  1.1%
Gln (Q)  60	  4.2%
Glu (E)  82	  5.8%
Gly (G)  55	  3.9%
His (H)  35	  2.5%
Ile (I) 101	  7.1%
Leu (L) 141	  9.9%
Lys (K) 102	  7.2%
Met (M)  33	  2.3%
Phe (F)  61	  4.3%
Pro (P)  87	  6.1%
Ser (S) 137	  9.6%
Thr (T)  78	  5.5%
Trp (W)  12	  0.8%
Tyr (Y)  41	  2.9%
Val (V)  66	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 177
Total number of positively charged residues (Arg + Lys): 140

Atomic composition:

Carbon      C	      7109
Hydrogen    H	     11148
Nitrogen    N	      1894
Oxygen      O	      2207
Sulfur      S	        48

Formula: C7109H11148N1894O2207S48
Total number of atoms: 22406

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   127965
Abs 0.1% (=1 g/l)   0.800, assuming all pairs of Cys residues form cystines


Ext. coefficient   127090
Abs 0.1% (=1 g/l)   0.794, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.03
This classifies the protein as unstable.



Aliphatic index: 84.52

Grand average of hydropathicity (GRAVY): -0.458