ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSDIRDVEE RNSRSSSSSS SSNSAAQSIG QHPYRGFDSE AAERVHELAR TLTSQSLLYT 

        70         80         90        100        110        120 
ANSNNSSSSN HNAHNADSRS VFSTDMEGVN PVFTNPDTPG YNPKLDPNSD QFSSTAWVQN 

       130        140        150        160        170        180 
MANICTSDPD FYKPYSLGCV WKNLSASGDS ADVSYQSTFA NIVPKLLTKG LRLLKPSKEE 

       190        200        210        220        230        240 
DTFQILKPMD GCLNPGELLV VLGRPGSGCT TLLKSISSNS HGFKIAKDSI VSYNGLSSSD 

       250        260        270        280        290        300 
IRKHYRGEVV YNAESDIHLP HLTVYQTLFT VARMKTPQNR IKGVDREAYA NHVTEVAMAT 

       310        320        330        340        350        360 
YGLSHTRDTK VGNDLVRGVS GGERKRVSIA EVAICGARFQ CWDNATRGLD SATALEFIRA 

       370        380        390        400        410        420 
LKTQADIGKT AATVAIYQCS QDAYDLFDKV CVLDDGYQLY FGPAKDAKKY FQDMGYYCPP 

       430        440        450        460        470        480 
RQTTADFLTS ITSPTERIIS KEFIEKGTRV PQTPKDMAEY WLQSESYKNL IKDIDSTLEK 

       490        500        510        520        530        540 
NTDEARNIIR DAHHAKQAKR APPSSPYVVN YGMQVKYLLI RNFWRMKQSA SVTLWQVIGN 

       550        560        570        580        590        600 
SVMAFILGSM FYKVMKKNDT STFYFRGAAM FFAILFNAFS CLLEIFSLYE TRPITEKHRT 

       610        620        630        640        650        660 
YSLYHPSADA FASVLSEMPP KLITAVCFNI IFYFLVDFRR NGGVFFFYFL INVIATFTLS 

       670        680        690        700        710        720 
HLFRCVGSLT KTLQEAMVPA SMLLLAISMY TGFAIPKTKI LGWSIWIWYI NPLAYLFESL 

       730        740        750        760        770        780 
MINEFHDRRF PCAQYIPAGP AYQNITGTQR VCSAVGAYPG NDYVLGDDFL KESYDYEHKH 

       790        800        810        820        830        840 
KWRGFGIGMA YVVFFFFVYL ILCEYNEGAK QKGEMVVFLR SKIKQLKKEG KLQEKHRPGD 

       850        860        870        880        890        900 
IENNAGSSPD SATTEKKILD DSSEGSDSSS DNAGLGLSKS EAIFHWRDLC YDVPIKGGQR 

       910        920        930        940        950        960 
RILNNVDGWV KPGTLTALMG ASGAGKTTLL DCLAERVTMG VITGNIFVDG RLRDESFPRS 

       970        980        990       1000       1010       1020 
IGYCQQQDLH LKTATVRESL RFSAYLRQPS SVSIEEKNRY VEEVIKILEM QQYSDAVVGV 

      1030       1040       1050       1060       1070       1080 
AGEGLNVEQR KRLTIGVELA ARPKLLVFLD EPTSGLDSQT AWDTCQLMRK LATHGQAILC 

      1090       1100       1110       1120       1130       1140 
TIHQPSAILM QQFDRLLFLQ KGGQTVYFGD LGEGCKTMID YFESKGAHKC PPDANPAEWM 

      1150       1160       1170       1180       1190       1200 
LEVVGAAPGS HATQDYNEVW RNSDEYKAVQ EELDWMEKNL PGRSKEPTAE EHKPFAASLY 

      1210       1220       1230       1240       1250       1260 
YQFKMVTIRL FQQYWRSPDY LWSKFILTIF NQVFIGFTFF KADRSLQGLQ NQMLSIFMYT 

      1270       1280       1290       1300       1310       1320 
VIFNPILQQY LPSFVQQRDL YEARERPSRT FSWLAFFLSQ IIVEIPWNIL AGTIAYCIYY 

      1330       1340       1350       1360       1370       1380 
YAVGFYANAS AAGQLHERGA LFWLFSIAFY VYIGSMGLLM ISFNEVAETA AHMGTLLFTM 

      1390       1400       1410       1420       1430       1440 
ALSFCGVMAT PKVMPRFWIF MYRVSPLTYM IDALLALGVA NVDVKCSNYE MVKFTPPSGT 

      1450       1460       1470       1480       1490       1500 
TCGDYMASYI KLAGTGYLSD PSATDICSFC AVSTTNAFLA TFSSHYYRRW RNYGIFICYI 

      1510       1520 
AFDYIAATFL YWLSRVPKKN GKISEKPKK 

References and documentation are available.

Number of amino acids: 1529

Molecular weight: 172255.74

Theoretical pI: 8.18

Amino acid composition: 
Ala (A) 121	  7.9%
Arg (R)  73	  4.8%
Asn (N)  63	  4.1%
Asp (D)  77	  5.0%
Cys (C)  29	  1.9%
Gln (Q)  60	  3.9%
Glu (E)  73	  4.8%
Gly (G)  96	  6.3%
His (H)  29	  1.9%
Ile (I)  88	  5.8%
Leu (L) 130	  8.5%
Lys (K)  84	  5.5%
Met (M)  42	  2.7%
Phe (F)  88	  5.8%
Pro (P)  64	  4.2%
Ser (S) 132	  8.6%
Thr (T)  90	  5.9%
Trp (W)  24	  1.6%
Tyr (Y)  78	  5.1%
Val (V)  88	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 150
Total number of positively charged residues (Arg + Lys): 157

Atomic composition:

Carbon      C	      7775
Hydrogen    H	     11921
Nitrogen    N	      2037
Oxygen      O	      2253
Sulfur      S	        71

Formula: C7775H11921N2037O2253S71
Total number of atoms: 24057

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   249970
Abs 0.1% (=1 g/l)   1.451, assuming all pairs of Cys residues form cystines


Ext. coefficient   248220
Abs 0.1% (=1 g/l)   1.441, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.47
This classifies the protein as unstable.



Aliphatic index: 80.21

Grand average of hydropathicity (GRAVY): -0.171