ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSDLAAELG FDPALKKKKK TKKVIPDDFD AAVNGKENGS GDDLFAGLKK KKKKSKSVSA 

        70         80         90        100        110        120 
DAEAEKEPTD DIAEALGELS LKKKKKKTKD SSVDAFEKEL AKAGLDNVDA ESKEGTPSAN 

       130        140        150        160        170        180 
SSIQQEVGLP YSELLSRFFN ILRTNNPELA GDRSGPKFRI PPPVCLRDGK KTIFSNIQDI 

       190        200        210        220        230        240 
AEKLHRSPEH LIQYLFAELG TSGSVDGQKR LVIKGKFQSK QMENVLRRYI LEYVTCKTCK 

       250        260        270        280 
SINTELKREQ SNRLFFMVCK SCGSTRSVSS IKTGFQATVG KRRRM 

References and documentation are available.

Number of amino acids: 285

Molecular weight: 31574.19

Theoretical pI: 9.53

Amino acid composition: 
Ala (A)  20	  7.0%
Arg (R)  15	  5.3%
Asn (N)  11	  3.9%
Asp (D)  18	  6.3%
Cys (C)   5	  1.8%
Gln (Q)   9	  3.2%
Glu (E)  21	  7.4%
Gly (G)  19	  6.7%
His (H)   2	  0.7%
Ile (I)  13	  4.6%
Leu (L)  25	  8.8%
Lys (K)  40	 14.0%
Met (M)   4	  1.4%
Phe (F)  13	  4.6%
Pro (P)  11	  3.9%
Ser (S)  28	  9.8%
Thr (T)  13	  4.6%
Trp (W)   0	  0.0%
Tyr (Y)   4	  1.4%
Val (V)  14	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      1383
Hydrogen    H	      2274
Nitrogen    N	       394
Oxygen      O	       429
Sulfur      S	         9

Formula: C1383H2274N394O429S9
Total number of atoms: 4489

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     6210
Abs 0.1% (=1 g/l)   0.197, assuming all pairs of Cys residues form cystines


Ext. coefficient     5960
Abs 0.1% (=1 g/l)   0.189, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.36
This classifies the protein as unstable.



Aliphatic index: 73.26

Grand average of hydropathicity (GRAVY): -0.679