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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSEDKKPVV DKKEEAAPKP PSSAVFSMFG GKKAEKPETK KDEEDTKEET KKEGDDAPES

70 80 90 100 110 120
PDIHFEPVVH LEKVDVKTME EDEEVLYKVR AKLFRFDADA KEWKERGTGD CKFLKNKKTN

130 140 150 160 170 180
KVRILMRRDK TLKICANHII APEYTLKPNV GSDRSWVYAC TADIAEGEAE AFTFAIRFGS

190 200
KENADKFKEE FEKAQEINKK A


References and documentation are available.

Number of amino acids: 201 Molecular weight: 22952.99 Theoretical pI: 5.85
Amino acid composition: 
Ala (A) 19 9.5% Arg (R) 8 4.0% Asn (N) 6 3.0% Asp (D) 16 8.0% Cys (C) 3 1.5% Gln (Q) 1 0.5% Glu (E) 28 13.9% Gly (G) 8 4.0% His (H) 3 1.5% Ile (I) 8 4.0% Leu (L) 7 3.5% Lys (K) 33 16.4% Met (M) 4 2.0% Phe (F) 11 5.5% Pro (P) 10 5.0% Ser (S) 9 4.5% Thr (T) 10 5.0% Trp (W) 2 1.0% Tyr (Y) 3 1.5% Val (V) 12 6.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 44 Total number of positively charged residues (Arg + Lys): 41 Atomic composition: Carbon C 1014 Hydrogen H 1609 Nitrogen N 273 Oxygen O 319 Sulfur S 7 Formula: C1014H1609N273O319S7 Total number of atoms: 3222 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 15595 Abs 0.1% (=1 g/l) 0.679, assuming all pairs of Cys residues form cystines Ext. coefficient 15470 Abs 0.1% (=1 g/l) 0.674, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 44.28 This classifies the protein as unstable. Aliphatic index: 55.87 Grand average of hydropathicity (GRAVY): -0.989