ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSEDKKPVV DKKEEAAPKP PSSAVFSMFG GKKAEKPETK KDEEDTKEET KKEGDDAPES
70 80 90 100 110 120
PDIHFEPVVH LEKVDVKTME EDEEVLYKVR AKLFRFDADA KEWKERGTGD CKFLKNKKTN
130 140 150 160 170 180
KVRILMRRDK TLKICANHII APEYTLKPNV GSDRSWVYAC TADIAEGEAE AFTFAIRFGS
190 200
KENADKFKEE FEKAQEINKK A
References and
documentation are available.
Number of amino acids: 201
Molecular weight: 22952.99
Theoretical pI: 5.85
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1014
Hydrogen H 1609
Nitrogen N 273
Oxygen O 319
Sulfur S 7
Formula: C
1014H
1609N
273O
319S
7
Total number of atoms: 3222
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15595
Abs 0.1% (=1 g/l) 0.679, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.674, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.28
This classifies the protein as unstable.
Aliphatic index: 55.87
Grand average of hydropathicity (GRAVY): -0.989