ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSEDYGYDY DLLFKIVLIG DSGVGKSNLL SRFTKNEFNM DSKSTIGVEF ATRTLEIDGK 

        70         80         90        100        110        120 
RIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS YENCNHWLSE LRENADDNVA 

       130        140        150        160        170        180 
VGLIGNKSDL AHLRAVPTEE SKTFAQENQL LFTETSALNS ENVDKAFEEL INTIYQKVSK 

       190        200        210        220 
HQMDLGDSSA NGNANGASAP NGPTISLTPT PNENKKANGN NCC 

References and documentation are available.

Number of amino acids: 223

Molecular weight: 24469.04

Theoretical pI: 4.97

Amino acid composition: 
Ala (A)  19	  8.5%
Arg (R)   8	  3.6%
Asn (N)  21	  9.4%
Asp (D)  14	  6.3%
Cys (C)   3	  1.3%
Gln (Q)   6	  2.7%
Glu (E)  16	  7.2%
Gly (G)  16	  7.2%
His (H)   3	  1.3%
Ile (I)  13	  5.8%
Leu (L)  18	  8.1%
Lys (K)  14	  6.3%
Met (M)   3	  1.3%
Phe (F)   7	  3.1%
Pro (P)   5	  2.2%
Ser (S)  22	  9.9%
Thr (T)  14	  6.3%
Trp (W)   2	  0.9%
Tyr (Y)   9	  4.0%
Val (V)  10	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1062
Hydrogen    H	      1666
Nitrogen    N	       296
Oxygen      O	       356
Sulfur      S	         6

Formula: C1062H1666N296O356S6
Total number of atoms: 3386

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24535
Abs 0.1% (=1 g/l)   1.003, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.998, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 25.55
This classifies the protein as stable.



Aliphatic index: 75.74

Grand average of hydropathicity (GRAVY): -0.534