ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSEEMLYAQ GAKIWVPHAD LVWESATLEE SYRKGAGFLK ICTDSGKLKE VKLKADGSDL 

        70         80         90        100        110        120 
PPLRNPAILV GQNDLTTLSY LHEPGVLHNL RVRFCERQII YTYCGIILVA INPYAEMPLY 

       130        140        150        160        170        180 
GPSIIRAYRG HAMGDLEPHI FALAEEAYTK LERENCNLSI IVSGESGAGK TVSAKYAMRY 

       190        200        210        220        230        240 
FAAVGGSESE TQVERKVLAS SPIMEAFGNA KTTRNDNSSR FGKFTKLLFR NQMGVMFLQG 

       250        260        270        280        290        300 
ATMHTYLLEK SRVVYQAQGE RNYHIFYQLC AARSKYPELV LDHQDKFQFL NMGGAPEIER 

       310        320        330        340        350        360 
VSDAEQFNET VQAMTVLGFS IQQIADIVKI LAGILHLGNI QVSKKFNEGS EEEDSDSCDI 

       370        380        390        400        410        420 
FHNDIHLQIT ADLLRVSADD LRRWLLMRKI ESVNEYVLIP NSIEAAQAAR DALAKHIYAK 

       430        440        450        460        470        480 
LFQYIVGVLN KSLNNGSKQC SFIGVLDIYG FETFEVNSFE QFCINYANEK LQQQFNQHVF 

       490        500        510        520        530        540 
KLEQEEYLKE GITWTMIDFY DNQPCIDLIE SRLGVLDLLD EECRMPKGSD ESWAGKLIGK 

       550        560        570        580        590        600 
CNKFPHFEKP RFGTTSFFIK HFSDTVEYDV NGFLEKNRDT VSKELTQVLS ESNMSLAKQV 

       610        620        630        640        650        660 
MTLEEIDTLC VDSAKSSTLG GRVVISAGRK QQGNDTRRRV VPSKQHRKTV GSQFQESLAS 

       670        680        690        700        710        720 
LISTLHATTP HYVRCIKPND DKVAFKWETA KIIQQLRACG VLETVRISAA GFPSRWLYPD 

       730        740        750        760        770        780 
FYMRYQLLVY RSKLDKNDMK LSCRNIVMKW IQDEDKYRFG NTQIFFRAGQ VAFLEQVRAN 

       790        800        810        820        830        840 
LRKKYITIVQ SVVRRFVYRR QFLRIQKVIN GIQKHARGYL ARERTQKMRE ARAGLILSKY 

       850        860        870        880        890        900 
ARGWLCRRRY LRLRHSISGI QTYARGMLAR NKFHAMRDHY RAVQIQRFVR GALARRAYQK 

       910        920        930        940        950        960 
RRRNIIICQA AIRRFLARRK FKRMKAEAKT ISHMENKYMG LENKIISMQQ RIDELNRDNS 

       970        980        990       1000       1010       1020 
NLKHKTSEIS VLKMKLELKK TLEAEFKNVK AACQDKDKLI EALNKQLEAE RDEKMQLLEE 

      1030       1040       1050       1060       1070       1080 
NGHAQEEWIS QKQTWRQENE ELRRQIDEII DMAKNAEVNQ RNQEDRMLAE IDNRELNEAY 

      1090       1100       1110       1120       1130       1140 
QRAIKDKEVI ENENFMLKEE LSRLTAGSFS LHARKASNAS SQNEDDVGYA SAKNTLDINR 

      1150       1160       1170       1180       1190       1200 
PPDLLSKNYS YNDSTSLVVK LRSILEEEKQ KHKVLQEQYI KLSSRHKPTE DSFRVSELEV 

      1210       1220       1230       1240       1250       1260 
ENEKLRSEYD QLRTSIKHGV EINELNAQHA ALQEEVRRRR EECIQLKAVL LQQSQSMRSL 

      1270       1280       1290       1300       1310       1320 
EPESLQMRGN DVNELMEAFH SQKLINRQLE SELKAITEEH NSKLVEMTQE IERLNNEKDE 

      1330       1340       1350       1360       1370       1380 
LQKVMFESID EFEDSNVDTL RQNDRYLRRE LQKAVAQFLL VQEELKLANA KLKAYRQDGG 

      1390       1400       1410       1420       1430       1440 
QLEHKIEEEM IRNKSNGTSA DVGANVTKQK SQNPQGLMKF HSSDLDKILQ RLLSALTPRT 

      1450       1460       1470       1480       1490       1500 
VVGLLPGFPA YLIFMCIRYT DLTNADDDVR ELLSKFVIQI KKMHRTPHPI ENRVIWLVNS 

      1510       1520       1530       1540       1550       1560 
ITLLNLMKQY GDVDEYVKFN TEKQNQQQLK NFNLFEYRRV ILDLIVNLYQ ALIMQIQGLL 

      1570       1580       1590       1600       1610       1620 
DPKIVPAILN NDEIQRGRQA HGMRSRATSI GASSSPEHGG GPAWKQLIGQ LEHFYKQFQH 

      1630       1640       1650       1660       1670       1680 
FGLDNCYAEQ IFHQLLYFIC AVALNCLMLR GDICMWETGM IIRYNIGCIE DWVRSKKMSN 

      1690       1700       1710       1720       1730       1740 
DVLTALAPLN QVSQLLQSRK SEQDVQTICD LCTSLSTAQV LKVMKSYKLD DYESEITNVF 

      1750       1760       1770       1780       1790       1800 
LEKLTEKLNA RQMQKSNSDE FTIDQKFIQP FKVVFRYSDI KLEDIELPSH LNLDEFLTKI 

References and documentation are available.

Number of amino acids: 1800

Molecular weight: 208245.39

Theoretical pI: 8.67

Amino acid composition: 
Ala (A) 118	  6.6%
Arg (R) 125	  6.9%
Asn (N)  97	  5.4%
Asp (D)  89	  4.9%
Cys (C)  27	  1.5%
Gln (Q) 117	  6.5%
Glu (E) 149	  8.3%
Gly (G)  80	  4.4%
His (H)  42	  2.3%
Ile (I) 118	  6.6%
Leu (L) 187	 10.4%
Lys (K) 131	  7.3%
Met (M)  49	  2.7%
Phe (F)  72	  4.0%
Pro (P)  39	  2.2%
Ser (S) 118	  6.6%
Thr (T)  69	  3.8%
Trp (W)  15	  0.8%
Tyr (Y)  60	  3.3%
Val (V)  98	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 238
Total number of positively charged residues (Arg + Lys): 256

Atomic composition:

Carbon      C	      9200
Hydrogen    H	     14701
Nitrogen    N	      2619
Oxygen      O	      2738
Sulfur      S	        76

Formula: C9200H14701N2619O2738S76
Total number of atoms: 29334

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   173525
Abs 0.1% (=1 g/l)   0.833, assuming all pairs of Cys residues form cystines


Ext. coefficient   171900
Abs 0.1% (=1 g/l)   0.825, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.49
This classifies the protein as unstable.



Aliphatic index: 88.43

Grand average of hydropathicity (GRAVY): -0.495