ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSEKAVPCY EESVRLLNGL QSNAATIKKL RVQRENLQAI NLPQCRKYLE SLNISAEDLN 

        70         80         90        100        110        120 
ALNIIHVSGT KGKGSACAFV ESILRSQGLR TGFYSSPHLV HVRERIQVDG QPVSEQMFAE 

       130        140        150        160        170        180 
EFFHVYDIIK REHSDNMPAY FKFLTLLAFR IFVKLNVQVM ILEVGIGGEY DCTNVVEKPK 

       190        200        210        220        230        240 
VCGVTTLDYD HMSILGNKLS EIAWHKAGIF KESVPAFYSP TTTEAEEVLI ARAISKHVPL 

       250        260        270        280        290        300 
FQTPPVSAYQ FARDISPGIR GAHQFSNVSM ALQLVRAWAE KCGFPLPGVP LSTDTSGFNV 

       310        320        330        340        350        360 
PLWMCDAIES CRWPGRSQIV STDRNVTYLL DGAHTPKSME ACSEWAAEEI VNLKKENVKK 

       370        380        390        400        410        420 
ILLFQCTADR CPSTLIKYLK PLGISQIVSC PTQLHSSIDK SADSANLNAS RDEQAEKANQ 

       430        440        450        460        470        480 
CVQAWKESLD QPESVTEDQM KVFDCISSAY KFIESQAASQ EILVLVTGSL HLVGGVLNLA 


GKGK 

References and documentation are available.

Number of amino acids: 484

Molecular weight: 53594.45

Theoretical pI: 6.33

Amino acid composition: 
Ala (A)  39	  8.1%
Arg (R)  19	  3.9%
Asn (N)  20	  4.1%
Asp (D)  19	  3.9%
Cys (C)  14	  2.9%
Gln (Q)  24	  5.0%
Glu (E)  34	  7.0%
Gly (G)  26	  5.4%
His (H)  12	  2.5%
Ile (I)  30	  6.2%
Leu (L)  45	  9.3%
Lys (K)  30	  6.2%
Met (M)   9	  1.9%
Phe (F)  19	  3.9%
Pro (P)  22	  4.5%
Ser (S)  45	  9.3%
Thr (T)  21	  4.3%
Trp (W)   6	  1.2%
Tyr (Y)  12	  2.5%
Val (V)  38	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2382
Hydrogen    H	      3775
Nitrogen    N	       645
Oxygen      O	       713
Sulfur      S	        23

Formula: C2382H3775N645O713S23
Total number of atoms: 7538

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51755
Abs 0.1% (=1 g/l)   0.966, assuming all pairs of Cys residues form cystines


Ext. coefficient    50880
Abs 0.1% (=1 g/l)   0.949, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.89
This classifies the protein as unstable.



Aliphatic index: 91.26

Grand average of hydropathicity (GRAVY): -0.117