ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSESPQDQP QKEQISNNVG VTTNSTSNEE TSRSQDDNVK EVNGNDDTKE EEQEEDAELD
70 80 90 100 110 120
DLFGDDNDDD DDDDVKKSET EKSDSDSDED DEGENINHRS RHRESLGLDD DEAEEQAMYT
130 140 150 160 170 180
RKFYGEDANN FSDQDETTHT FKEENVELVR HIIPSKANVN ETASHNEIFY ARIPNFLTID
190 200 210 220 230 240
PIPFDPPSFE AKVNERASNS ASREDQLDDR LIDENTVRWR YSRDKDQHVF KESNTQIVQW
250 260 270 280 290 300
SDGTYSLKVG EECTDILVND TSNTFLTVSH DQQELIQCYE GGEIKKTLMF IPTSTNSKIH
310 320 330 340 350 360
QKLSKAVIRR NQRQSKGPGT YIVSMDPEVE KKELERKQSQ ILRDRRRRQL KEKEKQESPD
370 380 390 400 410 420
AAFETGFRKQ NSPTTYGASR RNEYEEDDFL VDDDEEEEAA FDDEEDDNEE EEEEEDADEE
430 440 450 460
NASRLRNLKR EGAAMYREEE EEEKDRSETK RRRVAVIEDD EDED
References and
documentation are available.
Number of amino acids: 464
Molecular weight: 53920.11
Theoretical pI: 4.32
Total number of negatively charged residues (Asp + Glu): 133
Total number of positively charged residues (Arg + Lys): 62
Atomic composition:
Carbon C 2258
Hydrogen H 3517
Nitrogen N 663
Oxygen O 859
Sulfur S 7
Formula: C
2258H
3517N
663O
859S
7
Total number of atoms: 7304
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26025
Abs 0.1% (=1 g/l) 0.483, assuming all pairs of Cys residues form cystines
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 0.480, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 70.43
This classifies the protein as unstable.
Aliphatic index: 50.43
Grand average of hydropathicity (GRAVY): -1.477