ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSEYIIGRN PVIEALRSGR DINKIWIAEG AAKGQVQIVL ALAKENKIIL QHAPKKKLDQ 

        70         80         90        100        110        120 
LVEGNHQGVI AQVAAYQYAE LEDLFKVAEK RNEDPFFLIL DEIEDPHNLG SIMRTADATG 

       130        140        150        160        170        180 
AHGIIIPKRR AVGLTASVAK ASTGAIEYIP VARVTNLSRT IDELKERGLW IAGTDAKGKT 

       190        200        210        220        230        240 
DYRNLDGKMP IGLVIGSEGK GMSRIIGEKC DFLITLPMVG KVTSLNASVA ASLLMYEVYR 


KRNEIGK 

References and documentation are available.

Number of amino acids: 247

Molecular weight: 27007.33

Theoretical pI: 9.01

Amino acid composition: 
Ala (A)  26	 10.5%
Arg (R)  14	  5.7%
Asn (N)  10	  4.0%
Asp (D)  12	  4.9%
Cys (C)   1	  0.4%
Gln (Q)   7	  2.8%
Glu (E)  18	  7.3%
Gly (G)  22	  8.9%
His (H)   4	  1.6%
Ile (I)  26	 10.5%
Leu (L)  22	  8.9%
Lys (K)  20	  8.1%
Met (M)   6	  2.4%
Phe (F)   4	  1.6%
Pro (P)   8	  3.2%
Ser (S)  12	  4.9%
Thr (T)  10	  4.0%
Trp (W)   2	  0.8%
Tyr (Y)   7	  2.8%
Val (V)  16	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1201
Hydrogen    H	      1972
Nitrogen    N	       336
Oxygen      O	       354
Sulfur      S	         7

Formula: C1201H1972N336O354S7
Total number of atoms: 3870

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.793, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.793, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.48
This classifies the protein as stable.



Aliphatic index: 105.10

Grand average of hydropathicity (GRAVY): -0.112