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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSFYTVVGV FIVVSAMSVL FWIMAPKNNQ AVWRSTVILT LAMMFLMWAI TFLCQLHPLV

70
APRRSDLRPE FAE


References and documentation are available.

Number of amino acids: 73 Molecular weight: 8381.10 Theoretical pI: 9.30
Amino acid composition: 
Ala (A) 7 9.6% Arg (R) 4 5.5% Asn (N) 2 2.7% Asp (D) 1 1.4% Cys (C) 1 1.4% Gln (Q) 2 2.7% Glu (E) 2 2.7% Gly (G) 1 1.4% His (H) 1 1.4% Ile (I) 4 5.5% Leu (L) 8 11.0% Lys (K) 1 1.4% Met (M) 6 8.2% Phe (F) 6 8.2% Pro (P) 4 5.5% Ser (S) 6 8.2% Thr (T) 4 5.5% Trp (W) 3 4.1% Tyr (Y) 1 1.4% Val (V) 9 12.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 3 Total number of positively charged residues (Arg + Lys): 5 Atomic composition: Carbon C 391 Hydrogen H 605 Nitrogen N 95 Oxygen O 95 Sulfur S 7 Formula: C391H605N95O95S7 Total number of atoms: 1193 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 17990 Abs 0.1% (=1 g/l) 2.146, assuming all pairs of Cys residues form cystines Ext. coefficient 17990 Abs 0.1% (=1 g/l) 2.146, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 58.41 This classifies the protein as unstable. Aliphatic index: 109.45 Grand average of hydropathicity (GRAVY): 0.842