ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSGGAGEYN EDRHLLRSTD GDEVGIGGGE GDLDVESQSP AIRSGAGGVR DLFKHIDRRF 

        70         80         90        100        110        120 
SLSGRRLSFK RMENIRVDRE RHNPSSSSAF SAAGEEDGGG ISNLHSVDDR NDEYGFDEEV 

       130        140        150        160        170        180 
LGDSAPPEWA LLLIGCLIGV AAGICVAGFN KGVHVIHEWA WAGTPNEGAA WLRLQRLADT 

       190        200        210        220        230        240 
WHRILLIPVT GGVIVGMMHG LLEILDQIRQ SNSSQRQGLD FLAGIYPVIK AIQAAVTLGT 

       250        260        270        280        290        300 
GCSLGPEGPS VDIGKSCANG FALMMENNRE RRIALTAAGA ASGIASGFNA AVAGCFFAIE 

       310        320        330        340        350        360 
TVLRPLRAEN SPPFTTAMII LASVISSTVS NALLGTQSAF TVPSYDLKSA AELPLYLILG 

       370        380        390        400        410        420 
MLCGAVSVVF SRLVTWFTKS FDFIKDKFGL PAIVCPALGG LGAGIIALKY PGILYWGFTN 

       430        440        450        460        470        480 
VEEILHTGKS ASAPGIWLLA QLAAAKVVAT ALCKGSGLVG GLYAPSLMIG AAVGAVFGGS 

       490        500        510        520        530        540 
AAEIINRAIP GNAAVAQPQA YALVGMAATL ASMCSVPLTS VLLLFELTKD YRILLPLMGA 

       550        560        570        580        590        600 
VGLAIWVPSV ANQGKESDSS EGRSTGRGYS SLSPSERKTE GVWRHTDNAD SLELTVIENP 

       610        620        630        640        650        660 
DHNSFLDEET ILEDLKVMRV MSKNYVKVSS GTTLREARNI LKESHQNCIM VVDDDDFLAG 

       670        680        690        700        710        720 
ILTHGDIRRY LSNNASTILD ENTCPVSSVC TKKISYRGQE RGLLTCYPDA TVGVAKELME 

       730        740        750        760        770        780 
ARGVKQLPVV KRGEVIHKGK RRKLLGLLHY DSIWTFLRDE MSRRRSINDR RKDKEVGTNG 


H 

References and documentation are available.

Number of amino acids: 781

Molecular weight: 83548.54

Theoretical pI: 6.28

Amino acid composition: 
Ala (A)  77	  9.9%
Arg (R)  47	  6.0%
Asn (N)  29	  3.7%
Asp (D)  39	  5.0%
Cys (C)  13	  1.7%
Gln (Q)  15	  1.9%
Glu (E)  44	  5.6%
Gly (G)  83	 10.6%
His (H)  16	  2.0%
Ile (I)  49	  6.3%
Leu (L)  83	 10.6%
Lys (K)  30	  3.8%
Met (M)  17	  2.2%
Phe (F)  24	  3.1%
Pro (P)  29	  3.7%
Ser (S)  67	  8.6%
Thr (T)  36	  4.6%
Trp (W)  11	  1.4%
Tyr (Y)  16	  2.0%
Val (V)  56	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 77

Atomic composition:

Carbon      C	      3689
Hydrogen    H	      5903
Nitrogen    N	      1039
Oxygen      O	      1111
Sulfur      S	        30

Formula: C3689H5903N1039O1111S30
Total number of atoms: 11772

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    85090
Abs 0.1% (=1 g/l)   1.018, assuming all pairs of Cys residues form cystines


Ext. coefficient    84340
Abs 0.1% (=1 g/l)   1.009, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.68
This classifies the protein as unstable.



Aliphatic index: 96.57

Grand average of hydropathicity (GRAVY): 0.036