ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSGLVLENT ARRDALIAIE KKYQKIWAEE HQFEIDAPSI EDEPITMDSE ELHRTYPKFM 

        70         80         90        100        110        120 
SSMAYPYMNG VMHAGHCFTL SKVEFSIGFE RMNGKRALFP LGFHCTGMPI LACADKLKRE 

       130        140        150        160        170        180 
AELFGKNFDN VPAEEEEIKE ETPAEKDHED VTKFKAKKSK AAAKKGRGKY QFEIMLQLGI 

       190        200        210        220        230        240 
PREEIIKFAD AKYWLTYFPP LCESDCTSLG ARIDWRRSFV TTDANPYYDA FIRWQMNKLK 

       250        260        270        280        290        300 
AAGKIKFGER YTIYSEKDGQ ACMDHDRQSG EGVTPQEYIG VKIEALEFAD DAAKIIDSSS 

       310        320        330        340        350        360 
DLDKSKKFYF VAATLRPETM YGQTCCFVSP TIEYGIFDAG DSYFITTERA FKNMSYQKLT 

       370        380        390        400        410        420 
PKRGFYKPIV TVPGKAFIGT KIHAPQSVYP ELRILPMETV IATKGTGVVT CVPSNSPDDY 

       430        440        450        460        470        480 
ITTKDLLHKP EYYGIKPEWI DHEIVPIMHT EKYGDLTAKA IVEEKKIQSP KDKNLLAEAK 

       490        500        510        520        530        540 
KIAYKEDYYT GTMIYGPYKG EKVEQAKNKV KADMIAAGEA FVYNEPESQV MSRSGDDCIV 

       550        560        570        580        590        600 
SLEDQWYVDY GEESWKKQAI ECLEGMQLFA PEVKNAFEGV LDWLKNWAVC RTYGLGTRLP 

       610        620        630        640        650        660 
WDEKYLVESL SDSTIYQSFY TIAHLLFKDY YGNEIGPLGI SADQMTDEVF DYIFQHQDDV 

       670        680        690        700        710        720 
KNTNIPLPAL QKLRREFEYF YPLDVSISGK DLIPNHLTFF IYTHVALFPK KFWPKGIRAN 

       730        740        750        760        770        780 
GHLMLNNSKM SKSTGNFMTL EQTVEKFGAD AARIAFADAG DTVEDANFDE SNANAAILRL 

       790        800        810        820        830        840 
FNLKEWAEEI TKESNLRTGE ITDFFDIAFE HEMNALIEKT YEQYALTNYK NALKYGLFDF 

       850        860        870        880        890        900 
QAARDYYREA SGVMHKDLIA RYIETQALLL APIAPHFAEY IYREVLGNQT SVQNAKFPRA 

       910        920        930        940        950        960 
SKPVDKGVLA ALDYLRNLQR SIREGEGQAL KKKKGKSAEI DASKPVKLTL LISESFPEWQ 

       970        980        990       1000       1010       1020 
SQCVEIVRKL FSEQTLDDNK KVREHIEPKE MKRAMPFISL LKQRLANEKP EDVFERELQF 

      1030       1040       1050       1060       1070       1080 
SEIDTVKAAA RNVKKAAQAL KIAEFSAISF PYGAKTGKDI FTGEEVEIPP VTKIVENAVP 

      1090 
GNPGVVFQNI 

References and documentation are available.

Number of amino acids: 1090

Molecular weight: 124141.56

Theoretical pI: 5.61

Amino acid composition: 
Ala (A)  97	  8.9%
Arg (R)  41	  3.8%
Asn (N)  39	  3.6%
Asp (D)  64	  5.9%
Cys (C)  13	  1.2%
Gln (Q)  37	  3.4%
Glu (E)  99	  9.1%
Gly (G)  60	  5.5%
His (H)  20	  1.8%
Ile (I)  74	  6.8%
Leu (L)  77	  7.1%
Lys (K) 100	  9.2%
Met (M)  27	  2.5%
Phe (F)  59	  5.4%
Pro (P)  52	  4.8%
Ser (S)  56	  5.1%
Thr (T)  57	  5.2%
Trp (W)  13	  1.2%
Tyr (Y)  52	  4.8%
Val (V)  53	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 163
Total number of positively charged residues (Arg + Lys): 141

Atomic composition:

Carbon      C	      5612
Hydrogen    H	      8660
Nitrogen    N	      1442
Oxygen      O	      1658
Sulfur      S	        40

Formula: C5612H8660N1442O1658S40
Total number of atoms: 17412

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   149730
Abs 0.1% (=1 g/l)   1.206, assuming all pairs of Cys residues form cystines


Ext. coefficient   148980
Abs 0.1% (=1 g/l)   1.200, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.63
This classifies the protein as unstable.



Aliphatic index: 77.03

Grand average of hydropathicity (GRAVY): -0.435