ProtParam
User-provided sequence:
10 20 30 40 50 60
MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD
70 80 90 100 110 120
LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD GSKITLLLNE NDNDSIPHEY
130 140 150 160 170 180
DLELTKKVVE NEYVFTEQNL KKYQQRKKEL EADPEKQRQA YLKKQEREEE LKKKQQQQKR
190 200 210 220 230 240
RNNRKKFNHR VMTDRDGRDR YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR
250 260 270 280 290 300
RNIVKLNNKE RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL
310 320 330 340 350 360
DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT LRPEYKKLKE
370 380 390 400
EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV
References and
documentation are available.
Number of amino acids: 400
Molecular weight: 46605.45
Theoretical pI: 6.72
Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 72
Atomic composition:
Carbon C 2023
Hydrogen H 3259
Nitrogen N 587
Oxygen O 649
Sulfur S 13
Formula: C
2023H
3259N
587O
649S
13
Total number of atoms: 6531
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38975
Abs 0.1% (=1 g/l) 0.836, assuming all pairs of Cys residues form cystines
Ext. coefficient 38850
Abs 0.1% (=1 g/l) 0.834, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.59
This classifies the protein as unstable.
Aliphatic index: 70.17
Grand average of hydropathicity (GRAVY): -1.091