ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSIDNDSDV DLTEDLAVAK IVKENPVARK MVRYILSRGE SQNSIITRNK LQSVIHEAAR 

        70         80         90        100        110        120 
EENIAKPSFS KMFMDINAIL YNVYGFELQG LPSKNNMNAG GNGSNSNTNK SMPEPLGHRA 

       130        140        150        160        170        180 
QKFILLNNVP HSKNFDDFKI LQSAHTYEEL IVTGEYIGDD IASGTSNTLE SKLSTDRDLV 

       190        200        210        220        230        240 
YKGVLSVILC IVFFSKNNIL HQELIKFLET FGIPSDGSKI AILNITIEDL IKSLEKREYI 

       250        260        270        280        290        300 
VRLEEKSDTD GEVISYRIGR RTQAELGLES LEKLVQEIMG LEKEQTKSLH DDIIKSIGDS 


YSI 

References and documentation are available.

Number of amino acids: 303

Molecular weight: 33964.57

Theoretical pI: 5.23

Amino acid composition: 
Ala (A)  13	  4.3%
Arg (R)  12	  4.0%
Asn (N)  21	  6.9%
Asp (D)  19	  6.3%
Cys (C)   1	  0.3%
Gln (Q)   9	  3.0%
Glu (E)  26	  8.6%
Gly (G)  18	  5.9%
His (H)   6	  2.0%
Ile (I)  32	 10.6%
Leu (L)  30	  9.9%
Lys (K)  23	  7.6%
Met (M)   7	  2.3%
Phe (F)  10	  3.3%
Pro (P)   7	  2.3%
Ser (S)  31	 10.2%
Thr (T)  13	  4.3%
Trp (W)   0	  0.0%
Tyr (Y)   9	  3.0%
Val (V)  16	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1497
Hydrogen    H	      2418
Nitrogen    N	       404
Oxygen      O	       477
Sulfur      S	         8

Formula: C1497H2418N404O477S8
Total number of atoms: 4804

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.395, assuming all pairs of Cys residues form cystines


Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.395, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.22
This classifies the protein as unstable.



Aliphatic index: 99.41

Grand average of hydropathicity (GRAVY): -0.320