ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSIHEVVAL IEELYSPHPK HDVNQIQQSL QSIQKSEQGF HLANELLSDD KYSANVKYFG 

        70         80         90        100        110        120 
ALTLTVQLNT RGENDYETLW NVFRSNLLYL TKFSTLYVSN PNMYGQSLII IKKLMSNLSL 

       130        140        150        160        170        180 
IFTKINDPQL NNAGNENMIK QWNNPINTFI QLMSVQNQNI NADQLLLDSI NCSLTYEQLS 

       190        200        210        220        230        240 
QFVSLSQKHN ELALTFTEVI VEDLTKFQTK RHSMSQIHEV VHEHLYISTM ALINLNLTAQ 

       250        260        270        280        290        300 
AVFNPTVFDC ITAWINYISL TRSVSSSGRM DLSEIFQNLI DLMYQSTEGS DGYENAEKIL 

       310        320        330        340        350        360 
TIFGNVFAND PLLMSYDLRQ QIECIFLGVV RPDSGITDIS NKNSWMLQYM NYLVTNDFFS 

       370        380        390        400        410        420 
ELKELAICIV DFLQINTLSV CNKLFTNIQA ADNGQVQDEY IQEYIKVLLQ MTNFPLTPVL 

       430        440        450        460        470        480 
QEFFSVRMVD FWLDLSDAYT NLASETLRPN SIELSTQIFQ QLINIYLPKI SLSVKQRIIE 

       490        500        510        520        530        540 
EEGESTSVNE FEDFRNAVSD LAQSLWSILG NDNLTNVLID GMGQMPAASD ETLIIKDTDV 

       550        560        570        580        590        600 
LFRIETMCFV LNTILVDMTL SESPWIKNIV DANKFFNQNV ISVFQTGFQT SASTKVSQIL 

       610        620        630        640        650        660 
KLDFVRTSTT LIGTLAGYFK QEPFQLNPYV EALFQGLHTC TNFTSKNEQE KISNDKLEVM 

       670        680        690        700        710        720 
VIKTVSTLCE TCREELTPYL MHFISFLNTV IMPDSNVSHF TRTKLVRSIG YVVQCQVSNG 

       730        740        750        760        770        780 
PEEQAKYILQ LTNLLSGSIE HCLASSVQLQ EQQDYINCLL YCISELATSL IQPTEIIEND 

       790        800        810        820        830        840 
ALLQRLSEFQ SFWSSDPLQI RSKIMCTIDK VLDNSIYCKN SAFVEIGCLI VGKGLNLPDG 

       850        860        870        880        890        900 
EPYFLKYNMS EVMNFVLRHV PNCELATCLP YFVYLLEKLI SEFRKELTPQ EFDFMFEKIL 

       910        920        930        940        950        960 
LVYYDAYIIN DPDLLQMTIG FVNNVLDVKP GLAIGSKHWT SFILPQFLKL IPSREKFTIV 

       970        980        990       1000       1010       1020 
AVAKFWTKLI NNKKYNQEEL TTVRQQVSSI GGDLVYQIMY GLFHTQRSDL NSYTDLLRAL 

      1030       1040       1050       1060       1070       1080 
VAKFPIEARE WLVAVLPQIC NNPAGHEKFI NKLLITRGSR AAGNVILQWW LDCTTLPNYQ 


G 

References and documentation are available.

Number of amino acids: 1081

Molecular weight: 123530.70

Theoretical pI: 4.90

Amino acid composition: 
Ala (A)  43	  4.0%
Arg (R)  27	  2.5%
Asn (N)  81	  7.5%
Asp (D)  50	  4.6%
Cys (C)  20	  1.9%
Gln (Q)  69	  6.4%
Glu (E)  66	  6.1%
Gly (G)  37	  3.4%
His (H)  17	  1.6%
Ile (I)  88	  8.1%
Leu (L) 134	 12.4%
Lys (K)  50	  4.6%
Met (M)  27	  2.5%
Phe (F)  58	  5.4%
Pro (P)  34	  3.1%
Ser (S)  88	  8.1%
Thr (T)  70	  6.5%
Trp (W)  13	  1.2%
Tyr (Y)  39	  3.6%
Val (V)  70	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 116
Total number of positively charged residues (Arg + Lys): 77

Atomic composition:

Carbon      C	      5573
Hydrogen    H	      8707
Nitrogen    N	      1409
Oxygen      O	      1661
Sulfur      S	        47

Formula: C5573H8707N1409O1661S47
Total number of atoms: 17397

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   130860
Abs 0.1% (=1 g/l)   1.059, assuming all pairs of Cys residues form cystines


Ext. coefficient   129610
Abs 0.1% (=1 g/l)   1.049, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.51
This classifies the protein as unstable.



Aliphatic index: 102.85

Grand average of hydropathicity (GRAVY): -0.012