ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MSSIKIEFAV QMRRGDESYA GALRSALDGV GQVEIDTQEG RVIIQTQRPW SEIQDKIEAT 

        70         80         90        100        110        120 
GVRAVLSGFG GQSAVALINT TGSVVDKTPI QGVVRFTTIT ADKKPGVVVD GVVDGLSPGL 

       130        140        150        160        170        180 
HGLHIHESGD TSAGCSSVGE HYNPRQSPHG SPAAGAEERH AGDLGNIRAD ENGRATFRFV 

       190        200        210        220        230        240 
DPVLEVWDII GRAVVLTANA DDLGRGGNDQ SLIDGNSGER IACGIIARSA GILENFKRIC 

       250        260 
ACDGVTLWDE RNKPLAGKDR SQKL 

References and documentation are available.

Number of amino acids: 264

Molecular weight: 27922.21

Theoretical pI: 5.41

Amino acid composition: 
Ala (A)  24	  9.1%
Arg (R)  19	  7.2%
Asn (N)   9	  3.4%
Asp (D)  20	  7.6%
Cys (C)   4	  1.5%
Gln (Q)  11	  4.2%
Glu (E)  15	  5.7%
Gly (G)  34	 12.9%
His (H)   6	  2.3%
Ile (I)  20	  7.6%
Leu (L)  16	  6.1%
Lys (K)   9	  3.4%
Met (M)   2	  0.8%
Phe (F)   6	  2.3%
Pro (P)   9	  3.4%
Ser (S)  19	  7.2%
Thr (T)  13	  4.9%
Trp (W)   3	  1.1%
Tyr (Y)   2	  0.8%
Val (V)  23	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1202
Hydrogen    H	      1941
Nitrogen    N	       365
Oxygen      O	       389
Sulfur      S	         6

Formula: C1202H1941N365O389S6
Total number of atoms: 3903

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19730
Abs 0.1% (=1 g/l)   0.707, assuming all pairs of Cys residues form cystines


Ext. coefficient    19480
Abs 0.1% (=1 g/l)   0.698, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.43
This classifies the protein as unstable.



Aliphatic index: 87.54

Grand average of hydropathicity (GRAVY): -0.260